Zebrafish segmentation and pair-rule patterning.
Zebrafish segmentation and pair-rule patterning.
复制标题
斑马鱼分割和配对规则图案。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
C. Nüsslein
中科院分区：
文献类型：
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作者：
F. V. Eeden;S. Holley;P. Haffter;C. Nüsslein
在脊椎动物胚胎中，体节形式的近轴中胚层内的分割是明显的，体节是产生躯干和尾部的椎骨和肌肉的重复结构。在斑马鱼中，我们的遗传筛选确定了两组影响体节形成和模式的突变体。一类突变，fss型突变体，在体节发生过程中破坏前后体节边界的形成。然而，在这些突变体中，近轴中胚层内的分割并没有完全消除。不规则的体节边界在胚胎发育后期形成，引人注目的是，椎骨没有融合。在这里，我们表明，在这些突变体中的不规则体节边界的形成是依赖于第二组基因的活性，你型基因，其中包括声波你，斑马鱼同源的果蝇节极性基因，音刺猬。进一步表征的缺陷所造成的fss型突变，我们研究了它们对her1，斑马鱼同源的果蝇配对规则基因多毛的表达的影响。在野生型胚胎中，her1是一个动态的，重复的模式，非常相似的果蝇和赤拟谷盗同行，表明一对规则机制也在脊椎动物近轴中胚层的分割功能。我们发现fss型突变体具有异常的配对规则模式。虽然不能确定一个her1突变体，双突变体，废除大多数her1表达的分析表明，一个her1突变体可能不会显示一个配对规则表型类似于在果蝇中观察到的多毛表型。累积起来，我们的数据表明，斑马鱼同源的果蝇节极性基因和配对规则基因参与分割近轴中胚层。然而，这两组基因之间的关系在遗传层次结构内管理分割和精确的作用，他们在分割过程中发挥可能显着不同的两种生物体。
Segmentation in the vertebrate embryo is evident within the paraxial mesoderm in the form of somites, which are repeated structures that give rise to the vertebrae and muscle of the trunk and tail. In the zebrafish, our genetic screen identified two groups of mutants that affect somite formation and pattern. Mutations of one class, the fss-type mutants, disrupt the formation of the anterior-posterior somite boundaries during somitogenesis. However, segmentation within the paraxial mesoderm is not completely eliminated in these mutants. Irregular somite boundaries form later during embryogenesis and, strikingly, the vertebrae are not fused. Here, we show that formation of the irregular somite boundaries in these mutants is dependent upon the activity of a second group of genes, the you-type genes, which include sonic you, the zebrafish homologue of the Drosophila segment polarity gene, sonic hedgehog. Further to characterize the defects caused by the fss-type mutations, we examined their effects on the expression of her1, a zebrafish homologue of the Drosophila pair-rule gene hairy. In wild-type embryos, her1 is expressed in a dynamic, repeating pattern, remarkably similar to that of its Drosophila and Tribolium counterparts, suggesting that a pair-rule mechanism also functions in the segmentation of the vertebrate paraxial mesoderm. We have found that the fss-type mutants have abnormal pair-rule patterning. Although a her1 mutant could not be identified, analysis of a double mutant that abolishes most her1 expression suggests that a her1 mutant may not display a pair-rule phenotype analogous to the hairy phenotype observed in Drosophila. Cumulatively, our data indicate that zebrafish homologues of both the Drosophila segment polarity genes and pair-rule genes are involved in segmenting the paraxial mesoderm. However, both the relationship between these two groups of genes within the genetic heirarchy governing segmentation and the precise roles that they play during segmentation likely differ significantly between the two organisms.
斑马鱼中 engrailed 样抗原的表达多样性。
DOI：
10.1242/dev.112.3.821
发表时间：
1991
期刊：
Development (Cambridge, England)
影响因子：
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作者：
Hatta,K;Bremiller,R;Westerfield,M;Kimmel,CB
通讯作者：
Kimmel,CB