A new humanized mouse model of Epstein-Barr virus infection that reproduces persistent infection, lymphoproliferative disorder, and cell-mediated and humoral immune responses
A new humanized mouse model of Epstein-Barr virus infection that reproduces persistent infection, lymphoproliferative disorder, and cell-mediated and humoral immune responses
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DOI：
10.1086/590502
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发表时间：
2008-09-01
影响因子：
6.4
通讯作者：
Fujiwara, Shigeyoshi
中科院分区：
文献类型：
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作者：
Yajima, Misako;Imadome, Ken-ichi;Fujiwara, Shigeyoshi
在接受造血干细胞移植的NOD/Shi-scid/IL-2R γ (null)（NOG）小鼠中，功能正常的人类免疫系统，包括T淋巴细胞、B淋巴细胞和自然杀伤淋巴细胞得以重建。在这里，我们证明这些人源化小鼠可以重现eb病毒（EBV）在人类感染的关键方面。接种类似1 × 10(3) TD50（50%转化剂量）的EBV引起B细胞淋巴增生性疾病，其组织病理学结果和潜伏的EBV基因表达与免疫功能低下患者非常相似。低剂量病毒接种(
The functional human immune system, including T, B, and natural killer lymphocytes, is reconstituted in NOD/Shi-scid/IL-2R gamma(null)(NOG) mice that receive hematopoietic stem cell transplants. Here, we show that these humanized mice can recapitulate key aspects of Epstein-Barr virus (EBV) infection in humans. Inoculation with similar to 1 x 10(3) TD50 (50% transforming dose) of EBV caused B cell lymphoproliferative disorder, with histopathological findings and latent EBV gene expression remarkably similar to that in immunocompromised patients. Inoculation with a low dose of virus (