Physiological Responses and Expression Profile of NADPH Oxidase in Rice (Oryza Sativa) Seedlings under Different Levels of Submergence.
Physiological Responses and Expression Profile of NADPH Oxidase in Rice (Oryza Sativa) Seedlings under Different Levels of Submergence.
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DOI：
10.1186/s12284-016-0074-9
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发表时间：
2016-12
期刊：
影响因子：
--
通讯作者：
Yang CY
中科院分区：
文献类型：
--
作者：
Wu YS;Yang CY
全球气候变化导致的洪水是一个严重的问题，经常导致农作物产量下降。洪水多发地区的稻田经常被完全或部分淹没。浸没会对内部氧气供应、糖分状态和生存产生不利影响。完全淹没会给植物带来巨大的压力，主要是因为周围多余的水剥夺了它们某些基本资源，如氧气、二氧化碳和用于光合作用的光。为了更好地理解不同程度洪水下的相关机制，有必要进一步观察生理反应，识别相关的Rboh基因，并确定它们在淹没过程中的调控方式。本研究在部分和完全淹没处理下，株高、叶鞘伸长率和叶绿素a、b和总含量均发生了显着的生理变化。在完全淹没的情况下，衰老调节基因受到严重影响。此外，在不同程度的浸没下，细胞内氧化稳态因 H2O2 和 O2− 的过量产生而被破坏，从而影响细胞活力和抗氧化酶活性。定量 RT-PCR 分析表明，不同程度的浸没条件下，Rboh 基因参与了复杂的调控。我们的研究结果表明，OsRboh 基因的生理效应和转录水平对水稻幼苗不同程度的淹没呈现出不同的反应。细胞内有不同的机制来响应不同程度的淹没。最后我们讨论了 OsRboh 表达和 ROS 产生的调节作用，这对于维持体内平衡非常重要，有助于水稻幼苗面对不同程度的淹没。本文的在线版本 (doi:10.1186/s12284-016-0074-9) 包含补充材料，可供授权用户使用。
Flooding due to global climate change is a serious problem that frequently decreases crop yields. Rice fields in flood-prone areas often experience full or partial submergence. Submergence has an adverse effect on internal oxygen availability, sugar status and survival. Complete submergence imposes severe pressure on plants, principally because the excess water in their surroundings deprives them of certain basic resources such as oxygen, carbon dioxide and light for photosynthesis. To better understand the mechanisms involved under different levels of flooding, it is necessary to further observe physiological responses and to identify the Rboh genes involved and determine how they are regulated during submergence. In this study, significant physiological changes were observed in plant height, leaf sheath elongation and chlorophyll a, b and total content under partial and full submergence treatments. Senescence-regulating genes were severely affected under full submergence. Additionally, intracellular oxidative homeostasis was disrupted by overproduction of H2O2 and O2−, which affected cell viability and antioxidant enzyme activity, under different levels of submergence. Quantitative RT-PCR analyses revealed that complex regulation of Rboh genes is involved under different levels of submergence. Our results demonstrated that the effect of physiological and the transcript levels of OsRboh genes were presented different responses to different levels of submergence in rice seedlings. There have different mechanism in intracellular to response different levels of submergence. Finally we discuss effects of the regulation of OsRboh expression and ROS production which was important to maintain homeostasis to help rice seedlings face different levels of submergence. The online version of this article (doi:10.1186/s12284-016-0074-9) contains supplementary material, which is available to authorized users.