Improved hatching for in vitro quail embryo culture using surrogate eggshell and artificial vessel
Improved hatching for in vitro quail embryo culture using surrogate eggshell and artificial vessel
复制标题
使用代孕蛋壳和人造容器改进体外鹌鹑胚胎培养的孵化
DOI：
10.1046/j.1440-169x.1998.t01-2-00010.x
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发表时间：
1998
期刊：
影响因子：
4.6
通讯作者：
S. Iijima
中科院分区：
文献类型：
--
作者：
M. Kamihira;S. Oguchi;A. Tachibana;Yukiko Kitagawa;S. Iijima
禽类胚胎培养的建立对于分析鸡的发育过程和建立能在蛋中产生有用生物材料的转基因鸡具有重要意义。然而，培养胚胎的孵化率已经达到了∼的50%。我们认为，培养胚胎孵化率低是由于氧气和钙的供应有限所致。在以鸡蛋壳为培养容器的鹌鹑胚胎培养中，提高了培养中期的存活率，并通过富氧孵化了30%的胚胎。此外，在充氧的基础上添加乳酸钙，孵化率可提高到80%。在本研究中，使用透气性聚四氟乙烯膜设计了一种全人工的用于鹌鹑胚胎培养的容器。添加蛋壳细粉和乳酸钙后，雏鸡胚胎生长发育正常，孵化率达43%。虽然孵化率低于使用代理蛋壳培养的孵化率，但我们使用完全人工的容器孵化了一个禽类胚胎。
The establishment of avian embryonic culture is important both for the analysis of the developmental process and the establishment of transgenic chickens that produce useful biological materials in eggs. However, the hatchability of cultured embryos has been ∼50%. We identified that the low rate of hatchability of cultured embryos was caused by limited oxygen and calcium availability. In quail embryo culture using chicken eggshell as a culture vessel,viability in the middle stage of culture was improved and 30% of embryos were hatched by oxygen enrichment. Furthermore, hatchability increased to 80% by supplementation with calcium lactate in addition to oxygen aeration. In the present study, a fully artificial vessel for quail embryo culture was designed using a gas‐permeable Teflon membrane. By the addition of fine eggshell powder and calcium lactate, quail embryos grew and developed normally, and 43% of embryos hatched. Although the hatchability was lower than that of cultures using a surrogate eggshell, we achieved in hatching an avian embryo using a fully artificial vessel.