Natural infection of cattle with an atypical 'HoBi'-like pestivirus -: Implications for BVD control and for the safety of biological products
Natural infection of cattle with an atypical 'HoBi'-like pestivirus -: Implications for BVD control and for the safety of biological products
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DOI：
10.1051/vetres:2007012
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发表时间：
2007-05-01
影响因子：
4.4
通讯作者：
Belak, Sandor
中科院分区：
文献类型：
--
作者：
Stahl, Karl;Kampa, Jaruwan;Belak, Sandor
在泰国一项关于牛病毒性腹泻(BVD)流行病学的研究中，在一头小牛的血清中检测到一种鼠疫病毒。比较核苷酸序列分析表明，该病毒与最近从巴西采集的一批胎牛血清中首次分离到的非典型鼠疫病毒(D32/00_‘Hobi’)关系密切。从受感染牛群中收集的牛的血清进行病毒中和试验的结果显示，这些牛对非典型鼠疫病毒Hobi的抗体效价明显高于对牛病毒性腹泻病毒1和2或边界病病毒的抗体效价。因此，这些结果也支持分子分析的结果，并证明了一种类似霍比的鼠疫病毒已经被引入到该种群中，并正在人群中传播。这项研究首次报告了一种与这种非典型瘟疫病毒有关的病毒在牛身上的自然感染，并表明这组瘟疫病毒可能已经在牛种群中传播。这一发现对BVD的控制以及用胎牛血清生产的疫苗和其他生物制品的生物安全具有重要意义。因此，这些非典型的鼠疫病毒应包括在血清学检测中，任何旨在检测生物制品或动物中的鼠疫病毒的诊断检测方法都应测试其检测能力。
During a study on Bovine Viral Diarrhoea (BVD) epidemiology in Thailand, a pestivirus was detected in serum from a calf. Comparative nucleotide sequence analysis showed that this virus was closely related to a recently described atypical pestivirus (D32/00_ 'HoBi') that was first isolated from a batch of foetal calf serum collected in Brazil. The results from virus neutralisation tests performed on sera collected from cattle in the herd of the infected calf, showed that these cattle had markedly higher antibody titres against the atypical pestivirus 'HoBi' than against Bovine Viral Diarrhoea Virus types 1 and 2, or Border Disease Virus. The results also supported, consequently, the results from the molecular analysis, and demonstrated that a 'HoBi'- like pestivirus had been introduced to, and was now circulating in the herd. This study is the first to report a natural infection in cattle with a virus related to this atypical pestivirus, and it suggests that this group of pestiviruses may already be spread in cattle populations. The findings have implications for BVD control and for the biosafety of vaccines and other biological products produced with foetal calf serum. Consequently, these atypical pestiviruses should be included in serological assays, and any diagnostic assay aimed at detection of pestiviruses in biological products or animals should be tested for its ability to detect them.