Association of UNP, a ubiquitin-specific protease, with the pocket proteins pRb, p107 and p130
Association of UNP, a ubiquitin-specific protease, with the pocket proteins pRb, p107 and p130
复制标题
DOI：
10.1038/sj.onc.1204823
复制
发表时间：
2001-09-06
期刊：
影响因子：
8
通讯作者：
Gray, DA
中科院分区：
文献类型：
--
作者：
Blanchette, P;Gilchrist, CA;Gray, DA
小鼠UNP基因编码一种广泛表达的泛素特异性蛋白酶。预测的UNP蛋白序列具有病毒癌蛋白共同的基序，这些蛋白通过这些基序与视网膜母细胞瘤基因产物pRb以及相关的“口袋蛋白”p107和p130相互作用。我们已经探索了UNP与口袋蛋白相互作用的可能性，并在这里报告了这种关联可以在体外和细胞中检测到。UNP和Pocket蛋白的关联对定点突变很敏感，其方式直接类似于病毒癌蛋白中记录的那些。我们得出结论，在细胞内，UNP确实与pRb物理关联，也可以与p107和p130关联。
The murine Unp gene encodes a widely expressed ubiquitin-specific protease. The predicted sequence of the UNP protein features motifs common to viral oncoproteins through which these proteins interact with the retinoblastoma gene product pRb, as well as the related 'pocket proteins' p107 and p130. We have explored the possibility that UNP interacts with pocket proteins, and report here that such associations can be detected in vitro and in cells. Associations of UNP and pocket proteins are sensitive to site-directed mutations in a manner directly analogous to those documented in viral oncoproteins. We conclude that within cells UNP does physically associate with pRb, and can also associate with p107 and p130.