Photoactivated DNA analogs of substrates of the nucleotide excision repair system and their interaction with proteins of NER-competent extract of HeLa cells. Synthesis and application of long model DNA
Photoactivated DNA analogs of substrates of the nucleotide excision repair system and their interaction with proteins of NER-competent extract of HeLa cells. Synthesis and application of long model DNA
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DOI：
10.1134/s0006297911010159
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发表时间：
2011-01-01
影响因子：
2.8
通讯作者：
Lavrik, O. I.
中科院分区：
文献类型：
--
作者：
Evdokimov, A. N.;Petruseva, I. O.;Lavrik, O. I.
核苷酸切除修复(NER)底物的长线性DNA类似物已被合成。它们是137-聚体双链，在其内部位置含有核苷酸和大体积取代基，模拟病变，带有氟氯叠氮吡啶和荧光素基团，使用dCMP和Dump的4N和5C位的间隔基片段引入(Fap-DC-和Flu-Du-DNA)，以及含有苯并[a]芘-N-脱氧胍的(+)-顺式立体异构体的DNA(BP-DG-DNA，131bp)。研究了修饰后的DNA双链与NER活性HeLa提取液中蛋白质的相互作用。模型DNA在特定切割反应中的底物性质在Fap-DC-DNA系列中是不同的
Long linear DNA analogs of nucleotide excision repair (NER) substrates have been synthesized. They are 137-mer duplexes containing in their internal positions nucleotides with bulky substitutes imitating lesions with fluorochloroazidopyridyl and fluorescein groups introduced using spacer fragments at the 4N and 5C positions of dCMP and dUMP (Fap-dC- and Flu-dU-DNA) and DNA containing a (+)-cis-stereoisomer of benzo[a]pyrene-N2-deoxyguanidine (BP-dG-DNA, 131 bp). The interaction of the modified DNA duplexes with the proteins of NER-competent HeLa extract was investigated. The substrate properties of the model DNA in the reaction of specific excision were shown to vary in the series Fap-dC-DNA