Germinal vesicle position and meiotic maturation in mouse oocyte
Germinal vesicle position and meiotic maturation in mouse oocyte
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DOI：
10.1530/rep-07-0036
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发表时间：
2007-06-01
期刊：
影响因子：
3.8
通讯作者：
Maro, Bernard
中科院分区：
文献类型：
--
作者：
Brunet, Stephane;Maro, Bernard
在减数分裂成熟过程中，哺乳动物卵母细胞经历不对称分裂，这对于形成功能性配子至关重要。在各种生物体中，在m期之前细胞核的精确定位在不对称细胞分裂中起着重要作用。然而，在哺乳动物卵母细胞中，细胞核（或生发囊泡，GV）的位置在I前期阻滞中的作用尚未被研究。在这里，我们发现不正常的小鼠卵母细胞具有外周GV，而正常的卵母细胞主要表现为GV的中心位置。此时，GV的位置与卵母细胞完成减数分裂成熟的能力有关。此外，在老年小鼠卵母细胞中观察到GV定心效率较低，通过减数分裂进行的能力降低。因此，GV的位置可以作为卵母细胞质量的简单形态学标记。
During meiotic maturation, mammalian oocytes undergo an asymmetric division which is crucial for the formation of a functional gamete. In various organisms, accurate positioning of the nucleus before M-phase plays a major role in asymmetric cell divisions. However, the role of the position of the nucleus (or germinal vesicle, GV) during the prophase I arrest has not been investigated in mammalian oocytes. Here, we show that incompetent mouse oocytes possess a peripheral GV, while competent oocytes mainly exhibit a central position of the GV. At that time, the position of the GV correlates with the ability of the oocyte to complete meiotic maturation. Moreover, a lower efficiency in GV centering and a reduced ability to progress through meiosis are observed in oocytes from old mice. Thus, the position of the GV could be used as a simple morphological marker of oocyte quality.