Structure and expression of germline epsilon transcripts in human B cells induced by interleukin 4 to switch to IgE production.
Structure and expression of germline epsilon transcripts in human B cells induced by interleukin 4 to switch to IgE production.
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DOI：
10.1084/jem.172.2.463
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发表时间：
1990-08-01
期刊：
影响因子：
--
通讯作者：
de Vries JE
中科院分区：
文献类型：
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作者：
Gauchat JF;Lebman DA;Coffman RL;Gascan H;de Vries JE
白细胞介素4（IL-4）诱导的IgE产生与2.2-kb生产性ε-mRNA的出现一致，但在此之前是1.7-kb ε-RNA的合成。对该RNA 5'端的cDNA拷贝的分析表明，1.7-kb ε-RNA是一种生殖系α-免疫球蛋白重链转录物，其外显子定位在开关区的5'端。通过开关区域的转录已经被牵连在类转换的控制。然而，IL-4或克隆的CD 4 + T细胞能够诱导生殖细胞的转录本，而不诱导IgE合成，这两种信号都是必需的。这些结果表明，人类生殖系ε-RNA的诱导并不一定导致IgE合成，并且在类别转换中涉及额外的调节机制。
Interleukin 4 (IL-4)-induced IgE production coincides with the appearance of the 2.2-kb productive epsilon-mRNA, but is preceded by synthesis of a 1.7-kb epsilon-RNA. Analysis of cDNA copies of the 5' end of this RNA indicated that the 1.7-kb epsilon-RNA is a germline epsilon immunoglobulin heavy chain transcript with an exon mapping 5' to the switch region. Transcription through switch regions has been implicated in the control of class switching. However, IL-4 or cloned CD4+ T cells were able to induce germline epsilon transcripts without inducing IgE synthesis, for which both signals were required. These results indicate that induction of human germline epsilon-RNA does not necessarily result in IgE synthesis, and that additional regulatory mechanisms are involved in class switching.