Quantitative PCR Assay for Mycobacterium pseudoshottsii and Mycobacterium shottsii and Application to Environmental Samples and Fishes from the Chesapeake Bay
Quantitative PCR Assay for Mycobacterium pseudoshottsii and Mycobacterium shottsii and Application to Environmental Samples and Fishes from the Chesapeake Bay
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DOI：
10.1128/aem.01091-10
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发表时间：
2010-09-01
影响因子：
4.4
通讯作者：
Vogelbein, W. K.
中科院分区：
文献类型：
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作者：
Gauthier, D. T.;Reece, K. S.;Vogelbein, W. K.
切萨皮克湾的条纹鲈鱼（Morone saxatilis）目前正经历着一种非常高的分枝杆菌病流行率，这种分枝杆菌病与新描述的分枝杆菌属物种，假肖氏分枝杆菌和M. shottsii。这些分枝杆菌的生态外的条纹鲈鱼主机是目前未知的。在这项工作中，我们建立了M. pseudoshottsii和M. shottsii和应用这些分析的DNA提取物从切萨皮克湾的水和沉积物样品，以及组织从两个主要猎物的条纹鲈鱼，大西洋鲱鱼（Brevoortia bassnus）和海湾红鱼（Anchoa mitchili）。在切萨皮克湾的主茎水样中发现了普遍存在的假肖氏分枝杆菌，在弗吉尼亚州拉帕汉诺克河的水和沉积物中也发现了假肖氏分枝杆菌。M.在鲱鱼和鲱鱼组织中也检测到pseudoshottsii。相比之下，M。在水、沉积物或猎物鱼组织中未检测到shottsii。结合其非色素表型，这是经常发现的专性致病性分枝杆菌的人类，这种模式的发生表明，M。shottsii可能是条纹鲈专性病原菌。
Striped bass (Morone saxatilis) in the Chesapeake Bay are currently experiencing a very high prevalence of mycobacteriosis associated with newly described Mycobacterium species, Mycobacterium pseudoshottsii and M. shottsii. The ecology of these mycobacteria outside the striped bass host is currently unknown. In this work, we developed quantitative real-time PCR assays for M. pseudoshottsii and M. shottsii and applied these assays to DNA extracts from Chesapeake Bay water and sediment samples, as well as to tissues from two dominant prey of striped bass, Atlantic menhaden (Brevoortia tyrannus) and bay anchovy (Anchoa mitchilli). Mycobacterium pseudoshottsii was found to be ubiquitous in water samples from the main stem of the Chesapeake Bay and was also present in water and sediments from the Rappahannock River, Virginia. M. pseudoshottsii was also detected in menhaden and anchovy tissues. In contrast, M. shottsii was not detected in water, sediment, or prey fish tissues. In conjunction with its nonpigmented phenotype, which is frequently found in obligately pathogenic mycobacteria of humans, this pattern of occurrence suggests that M. shottsii may be an obligate pathogen of striped bass.