NICOTINAMIDE METHYLATION AND HEPATIC ENERGY RESERVE - A STUDY BY LIVER PERFUSION IN-VITRO
NICOTINAMIDE METHYLATION AND HEPATIC ENERGY RESERVE - A STUDY BY LIVER PERFUSION IN-VITRO
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DOI：
10.1016/0168-8278(95)80206-1
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发表时间：
1995-10-01
影响因子：
25.7
通讯作者：
BUDILLON, G
中科院分区：
文献类型：
--
作者：
CUOMO, R;PUMPO, R;BUDILLON, G
背景/目标：从烟酰胺合成吡啶核苷酸需要三磷酸腺苷。在人体中，当外源性烟酰胺在此合成中利用不良时，过量的烟酰胺会遵循耗散代谢途径并以 N-甲基烟酰胺的形式从尿液中排出。在人肝硬化中，在基础条件下和口服烟酰胺后，N-甲基烟酰胺血清水平高于正常水平。本研究的目的是验证大鼠肝脏体外灌注期间、正常条件下以及代谢应激消耗三磷酸腺苷后，N-甲基烟酰胺的产生与三磷酸腺苷的肝含量之间的关系。方法：通过用盐水预洗15分钟获得“压力”结果：应激肝脏中三磷酸腺苷在用盐水预清洗后减少38％，在营养灌注结束时减少80％，在对照肝脏中，相应的减少在用营养培养基预清洗后减少1％，在用相同培养基灌注结束时减少65％，在应激肝脏中烟酰胺腺嘌呤二核苷酸总减少分别为44％和56％，并且对照肝脏中分别为 19% 和 52%，大鼠肝脏营养性灌注 90 分钟时，正常肝脏的 N-甲基烟酰胺输出水平为 31.50+/-4.72 nmol/g，应激肝脏为 66.40+/-13.17 nmol/g（p
Background/Aims: The synthesis of pyridine nucleotides from nicotinamide requires adenosine triphosphate. In man when exogenous nicotinamide is poorly utilized in this synthesis, the excess follows a dissipative metabolic pathway and is excreted in urine as N-methylnicotinamide. In human cirrhosis N-methylnicotinamide serum levels are higher than normal, in basal condition and after nicotinamide oral load, The aim of this study was to verify N-methylnicotinamide production in relation to hepatic content of adenosine triphosphate during in vitro perfusion of rat liver, in normal conditions and after adenosine triphosphate depletion by metabolic stress.Methods: ''Stress'' was obtained by pre-washing with saline for 15 min before the perfusion with nutritive medium.Results: The adenosine triphosphate decrease in the stressed liver was 38% after pre-washing with saline and 80% at the end of nutritive perfusion, In control liver the corresponding decreases were 1% after pre-washing with nutritive medium and 65% at the end of perfusion with the same medium, The total nicotinamide adenine dinucleotide decreases were 44% and 56% in the stressed liver, and 19% and 52% in the control liver, The output levels of N-methylnicotinamide at 90 min of rat liver nutritive perfusion were 31.50+/-4.72 nmol/g for normal liver and 66.40+/-13.17 for stressed liver (p