Mobilization of Endothelial Progenitor Cells From Bone Marrow is Impaired in a Piglet Model of Acute Respiratory Distress Syndrome*
Mobilization of Endothelial Progenitor Cells From Bone Marrow is Impaired in a Piglet Model of Acute Respiratory Distress Syndrome*
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DOI：
10.1097/pcc.0b013e31828a7242
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发表时间：
2013-06
影响因子：
4.1
通讯作者：
Y. Qi;L. Qian;Bo Sun;Yuanyuan Wang;Lijuan Liu;Pan-pan Wu;Libo Sun
中科院分区：
文献类型：
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作者：
Y. Qi;L. Qian;Bo Sun;Yuanyuan Wang;Lijuan Liu;Pan-pan Wu;Libo Sun
目的：为了表征中度和重度肺损伤模型中内皮祖细胞动员的特征，我们假设中度和重度肺损伤之间内皮祖细胞水平和动员细胞因子存在差异。设计：前瞻性、随机对照实验研究。单位：大学研究实验中心。受试者：15头健康仔猪。干预措施：将仔猪随机分为对照组、中度肺损伤（急性肺损伤）组和重度肺损伤（急性呼吸窘迫综合征）组。通过静脉输注油酸建立肺损伤。对动物进行机械通气24-48小时，然后使动物脱离通气并护理至第7天。测量和主要结果：通过流式细胞术定量内皮祖细胞。24 h后，与对照组相比，急性肺损伤组外周血内皮祖细胞数量增加，而急性呼吸窘迫综合征组外周血内皮祖细胞数量无明显变化。急性肺损伤组CD 34 +KDR+、KDR+ CD 133+、CD 34 +KDR+ CD 133+细胞数均高于急性呼吸窘迫综合征组。在骨髓中，CD 34 +KDR+和KDR+ CD 133+细胞的数量在急性呼吸窘迫综合征动物中更大，但在24小时急性肺损伤动物中没有改变。此外，血浆基质细胞衍生因子-1和血管内皮生长因子浓度在急性肺损伤中高于急性呼吸窘迫综合征24小时。与急性肺损伤相比，急性呼吸窘迫综合征患者骨髓基质金属蛋白酶9和可溶性kit配体水平降低。急性肺损伤组肺组织中CD 34、KDR和肺基质细胞衍生因子-1 mRNA表达均高于急性呼吸窘迫综合征组。此外，肺组织中CD 34、KDR和CD 133信使RNA的表达与肺中基质细胞衍生因子-1相关。结论：在中度急性肺损伤中，内皮祖细胞从骨髓中快速释放到循环中，而在急性呼吸窘迫综合征中，内皮祖细胞动员受损。
Objectives: To characterize the endothelial progenitor cell mobilization in the models of moderate and severe lung injury, we hypothesized that there were differences in endothelial progenitor cell levels and mobilizing cytokines between moderate and severe lung injury. Design: Prospective, randomized, and controlled experimental study. Setting: University research laboratory center. Subjects: Fifteen healthy piglets. Interventions: Piglets were randomly allocated to control, moderate lung injury (acute lung injury), and severe lung injury (acute respiratory distress syndrome) groups. Lung injury was established by intravenous infusion of oleic acid. Animals were mechanically ventilated for 24–48 hours, and then animals were weaned from ventilation and cared for until day 7. Measurements and Main Results: Endothelial progenitor cells were quantified by flow cytometry. After 24 hours, the number of endothelial progenitor cells in peripheral blood increased in the acute lung injury group but was not altered in the acute respiratory distress syndrome group compared to the control group. The number of CD34+KDR+, KDR+CD133+, and CD34+KDR+CD133+ cells was higher in the acute lung injury group than in the acute respiratory distress syndrome group. In bone marrow, the number of CD34+KDR+ and KDR+CD133+ cells was greater in acute respiratory distress syndrome animals but not altered in acute lung injury animals at 24 hours. Furthermore, plasma stromal cell-derived factor-1 and vascular endothelial growth factor concentrations were higher in acute lung injury than in acute respiratory distress syndrome at 24 hours. Matrix metalloproteinase-9 and soluble kit ligand levels in bone marrow were reduced in acute respiratory distress syndrome compared with acute lung injury. Lung CD34, KDR, and lung stromal cell-derived factor-1 messenger RNA expression were higher in the acute lung injury group than in the acute respiratory distress syndrome group. Furthermore, the expression of CD34, KDR, and CD133 messenger RNA in lung tissue was correlated with stromal cell-derived factor-1 in the lung. Conclusions: There was a rapid release of endothelial progenitor cells from bone marrow into circulation in moderate acute lung injury, and endothelial progenitor cell mobilization was impaired in acute respiratory distress syndrome.