Modeling the time evolution of the nanoparticle-protein corona in a body fluid.
Modeling the time evolution of the nanoparticle-protein corona in a body fluid.
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DOI：
10.1371/journal.pone.0010949
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发表时间：
2010-06-03
期刊：
影响因子：
3.7
通讯作者：
Linse S
中科院分区：
文献类型：
--
作者：
Dell'Orco D;Lundqvist M;Oslakovic C;Cedervall T;Linse S
与生物流体接触的纳米颗粒与蛋白质和其他生物分子相互作用，从而形成动态冠，其组成因连续的蛋白质缔合和解离事件而随时间变化。最终达到平衡，在这一点上，持续的交换不会影响日冕的组成。我们开发了一个简单而有效的动态模型的纳米粒子蛋白质冠在体液中，即人体血浆。该模型预测的时间演变和平衡的电晕的基础上的亲和力，化学计量和速率常数的组成。应用于人血清白蛋白，高密度脂蛋白（HDL）和纤维蛋白原与70 nm的N-异丙基丙烯酰胺/N-叔丁基丙烯酰胺共聚物纳米粒子的相互作用，包括HDL的新的实验数据。这里提出的简单模型可以很容易地进行修改，以模仿纳米颗粒蛋白质冠与新的生物流体或隔室的相互作用，一旦新的数据将是可用的，从而打开新的应用在纳米毒性和纳米医学。
Nanoparticles in contact with biological fluids interact with proteins and other biomolecules, thus forming a dynamic corona whose composition varies over time due to continuous protein association and dissociation events. Eventually equilibrium is reached, at which point the continued exchange will not affect the composition of the corona. We developed a simple and effective dynamic model of the nanoparticle protein corona in a body fluid, namely human plasma. The model predicts the time evolution and equilibrium composition of the corona based on affinities, stoichiometries and rate constants. An application to the interaction of human serum albumin, high density lipoprotein (HDL) and fibrinogen with 70 nm N-iso-propylacrylamide/N-tert-butylacrylamide copolymer nanoparticles is presented, including novel experimental data for HDL. The simple model presented here can easily be modified to mimic the interaction of the nanoparticle protein corona with a novel biological fluid or compartment once new data will be available, thus opening novel applications in nanotoxicity and nanomedicine.