One day exposure to FGF-2 was sufficient for the regenerative repair of full-thickness defects of articular cartilage in rabbits
One day exposure to FGF-2 was sufficient for the regenerative repair of full-thickness defects of articular cartilage in rabbits
复制标题
DOI：
10.1016/j.joca.2004.07.003
复制
发表时间：
2004-10-01
影响因子：
7
通讯作者：
Hiraki, Y
中科院分区：
文献类型：
--
作者：
Chuma, H;Mizuta, H;Hiraki, Y
目的：给药成纤维细胞生长因子（FGF）-2 2 2周诱导兔5 mm全层关节软骨缺损成功的软骨修复反应。本研究的目的是探讨短时间暴露于FGF-2对defects.Methods修复的影响：5 mm直径的圆柱形缺损，不自发修复，在兔膝关节股骨trophila创建。在最初的1天、3天或2周内，通过渗透泵向缺损处给予无菌盐水或FGF-2（150 pg/h），我们评估了FGF-2对修复组织中间充质细胞增殖和迁移的作用。共使用126只兔子，我们进行了三组实验。我们还研究了FGF-2对骨髓间充质细胞迁移的影响在vitro.Results：FGF-2处理1天或3天诱导的顺序软骨修复反应，导致成功的软骨表面重建的缺陷在8周内，以及2周的治疗没有。我们通过放射性同位素研究证实，注射的FGF-2从缺损处迅速消除（30分钟内残留率为50%）。FGF-2对培养的骨髓源性细胞的影响表明，FGF-2促进了动员和迁移的复制间充质细胞从骨髓.Conclusions：只有1天暴露于FGF-2是足够的诱导软骨修复反应在5毫米直径的全层缺损的关节软骨在兔。FGF-2刺激间充质细胞募集到缺损中，这是诱导软骨的限制步骤。(C)2004年国际骨关节炎研究学会。由爱思唯尔有限公司出版。保留所有权利。
Objectives: Administration of fibroblast growth factor (FGF)-2 for 2 weeks induces a successful cartilaginous repair response in 5-mm full-thickness articular cartilage defects in rabbits. The purpose of this study was to investigate the effects of a short time exposure to FGF-2 on the repair of the defects.Methods: Five-mm-diameter cylindrical defects, which do not repair spontaneously, were created in the femoral trochlea of the rabbit knees. The defects were administered sterile saline or FGF-2 (150 pg/h) via an osmotic pump for the initial 1 day, 3 days, or 2 weeks, and we assessed the FGF-2 action on the proliferation and migration of mesenchymal cells in the reparative tissue. Using a total of 126 rabbits, we performed three sets of experiments. We also studied the effect of FGF-2 on migration of marrow-derived mesenchymal cells in vitro.Results: FGF-2 treatment for 1 day or 3 days induced the sequential chondrogenic repair responses that led to successful cartilaginous resurfacing of defects within 8 weeks as well as the 2-week treatment did. We confirmed by a radioisotope study that FGF-2 injected was rapidly eliminated from the defects (a residual ratio of 50% within 30 min). The effect of FGF-2 on cultured marrow-derived cells suggested that FGF-2 facilitated the mobilization and migration of replicating mesenchymal cells from bone marrow.Conclusions: Only 1 day exposure to FGF-2 is sufficient for induction of the chondrogenic repair response in 5-mm-diameter full-thickness defects of articular cartilage in rabbits. FGF-2 stimulated the recruitment of mesenchymal cells into the defects, which was a limiting step for the induction of cartilage. (C) 2004 OsteoArthritis Research Society International. Published by Elsevier Ltd. All rights reserved.