Three-dimensional digital mouse atlas using high-resolution MRI
Three-dimensional digital mouse atlas using high-resolution MRI
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DOI：
10.1006/dbio.2001.0189
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发表时间：
2001-04-15
影响因子：
2.7
通讯作者：
Jacobs, RE
中科院分区：
文献类型：
--
作者：
Dhenain, M;Ruffins, SW;Jacobs, RE
我们提出了一个基于显微磁共振成像的小鼠胚胎的原型数字图谱。该图集由三个模块组成：(1)怀孕6天至15.5天的固定胚胎(DPC)[泰勒期(TS)8至24]的图像；(2)13.5DPC(TS 22)小鼠的前部带注释的图集，其解剖结构被描绘并链接到说明性文件；以及(3)整个13.5DPC胚胎和特定器官系统的三维渲染。说明性文件包括对图像中每个体积元素处的结构的简要描述，以及到3D重建的链接，从而允许可视化孤立结构的形状。这些文件还可以包含或链接到其他类型的信息和数据，包括关于结构的详细解剖和生理信息，以及指向在线参考、结构之间的关系、时间特征(细胞谱系模式、大小和形状变化)以及基因表达模式(空间和时间)的详细信息。以D1x5/D1x6基因的表达模式为例。这一数字地图集提供了一种方法，可以将特定数据放在正常标本解剖的背景下，以3D形式分析信息，并检查不同类型信息之间的关系。(C)2001年学术出版社。
We present an archetypal digital atlas of the mouse embryo based on microscopic magnetic resonance imaging. The atlas is composed of three modules: (1) images of fixed embryos 6 to 15.5 days postconception (dpc) [Theiler Stages (TS) 8 to 24]; (2) an annotated atlas of the anterior portion of a 13.5 dpc (TS 22) mouse with anatomical structures delineated and linked to explanatory files; and (3) three-dimensional renderings of the entire 13.5 dpc embryo and specific organ systems. The explanatory files include brief descriptions of the structure at each volume element in the image and links to 3D reconstructions, allowing visualization of the shape of the isolated structures. These files can also contain or be linked to other types of information and data including detailed anatomical and physiological information about structures with pointers to online references, relationships between structures, temporal characteristics (cell lineage patterns, size, and shape changes), and gene expression patterns (both spatial and temporal). As an example, we have "painted" in the expression pattern of D1x5/D1x6 genes. This digital atlas provides a means to put specific data within the context of normal specimen anatomy, to analyze the information in 3D, and to examine relationships between different types of information. (C) 2001 Academic Press.