Oocyte cumulus complex quality and oviduct transportation velocity in systemic autoimmune disease model mice
Oocyte cumulus complex quality and oviduct transportation velocity in systemic autoimmune disease model mice
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DOI：
10.1177/15353702231160875
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发表时间：
2023-03
影响因子：
3.2
通讯作者：
M. Hosotani;O. Ichii;Takafumi Watanabe;Y. Kon
中科院分区：
文献类型：
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作者：
M. Hosotani;O. Ichii;Takafumi Watanabe;Y. Kon
卵母细胞通过输卵管的运输涉及纤毛上皮细胞和卵丘细胞之间的相互作用。为了确定卵丘-卵母细胞复合体（COCs）的质量是否改变COCs的运输特性，我们比较了COCs通过漏斗离体与收集自不同小鼠的漏斗和COCs的各种组合的运输速度（TVC）。我们使用年轻和老年C57 BL/6 N和MRL/MpJ，和MRL/MpJ-Fas lpr/lpr小鼠作为具有完整雌性生殖功能的品系和由于纤毛上皮的形态功能异常而表现出卵母细胞拾取功能障碍的系统性自身免疫性疾病模型。老年MRL品系的TVC小于老年C57 BL/6 N小鼠，表明衰老影响了MRL品系中COC的转运。老年MRL/MpJ-Fas lpr/lpr小鼠的TVC在所有检查的组合中最小，而当从其他品系收集漏斗或COC时，TVC加速。这些结果表明，COCs的转运特性不仅取决于漏斗部纤毛的功能，而且还取决于COCs本身的性质。
Oocyte transportation by the oviduct involves the interaction between ciliated epithelial cells and cumulus cells. To determine whether the quality of cumulus–oocyte complexes (COCs) changes the transportation property of COCs, we compared the transportation velocity of COCs (TVC) by the infundibulum ex vivo with various combinations of infundibula and COCs collected from different mice. We used young and aged C57BL/6N and MRL/MpJ, and MRL/MpJ-Fas lpr/lpr mice as the strains with intact female reproductive function and the systemic autoimmune disease model exhibiting oocyte pick-up dysfunction owing to the morphofunctional abnormality of ciliated epithelium, respectively. The TVC of aged MRL strains was less than that of aged C57BL/6N mice, suggesting that aging affects the transportation of COCs in MRL strains. The TVC of aged MRL/MpJ-Fas lpr/lpr mice was the least among all examined combinations, whereas the TVC accelerated when the infundibulum or COCs were collected from other strains. These results indicate that the transportation property of COCs is determined not only by the ciliary function in the infundibulum but also by the properties of COCs.