Foxtail Millet NF-Y Families: Genome-Wide Survey and Evolution Analyses Identified Two Functional Genes Important in Abiotic Stresses.
Foxtail Millet NF-Y Families: Genome-Wide Survey and Evolution Analyses Identified Two Functional Genes Important in Abiotic Stresses.
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谷子 NF-Y 家族：全基因组调查和进化分析确定了两个在非生物胁迫中重要的功能基因
DOI：
10.3389/fpls.2015.01142
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发表时间：
2015
影响因子：
5.6
通讯作者：
Xu ZS
中科院分区：
文献类型：
--
作者：
Feng ZJ;He GH;Zheng WJ;Lu PP;Chen M;Gong YM;Ma YZ;Xu ZS
核因子Y（Nuclear Factor Y，NF-Y）基因与植物的非生物胁迫有关。谷子（Setaria italica）是一种优良的抗逆作物，为研究NF-Y家族在非生物反应中的作用提供了动力。在本研究中，共39个NF-Y基因被确定在谷子。同线性分析表明，谷子NF-Y基因在植物进化过程中经历了快速扩增和强烈的纯化选择。谷子的从头转录组组装揭示了11个干旱上调的NF-Y基因。SiNF-YA 1和SiNF-YB 8在干旱和盐胁迫下在叶和/或根中高度活化。阿坝和H_2O_2在逆境胁迫下对SiNF-YA_1和SiNF-YB_8的诱导起积极作用。瞬时荧光素酶（LUC）表达分析表明，SiNF-YA 1和SiNF-YB 8在正常或胁迫条件下均能激活烟草（Nicotiana tobacam）NtERD 10、NtLEA 5、NtCAT、NtSOD或NtPOD启动子驱动的LUC基因。SiNF-YA 1的过表达通过激活逆境相关基因NtERD 10和NtCAT 1，并在逆境下维持相对稳定的相对含水量（RWC）以及叶绿素、超氧化物歧化酶（SOD）、过氧化物酶（POD）、过氧化氢酶（CAT）和过氧化氢酶（CAT）含量，增强转基因系的耐旱性和耐盐性。SiNF-YB 8调控NtSOD、NtPOD、NtLEA 5和NtERD 10的表达，并赋予转基因株系相对较高的RWC和叶绿素含量以及较低的MDA含量，从而使转基因株系在逆境下具有耐旱和耐渗透的能力。因此，SiNF-YA 1和SiNF-YB 8可以激活逆境相关基因，改善植物的生理性状，从而提高植物对非生物胁迫的耐受性。所有这些结果将有助于在未来的研究谷子NF-Y的功能表征。
It was reported that Nuclear Factor Y (NF-Y) genes were involved in abiotic stress in plants. Foxtail millet (Setaria italica), an elite stress tolerant crop, provided an impetus for the investigation of the NF-Y families in abiotic responses. In the present study, a total of 39 NF-Y genes were identified in foxtail millet. Synteny analyses suggested that foxtail millet NF-Y genes had experienced rapid expansion and strong purifying selection during the process of plant evolution. De novo transcriptome assembly of foxtail millet revealed 11 drought up-regulated NF-Y genes. SiNF-YA1 and SiNF-YB8 were highly activated in leaves and/or roots by drought and salt stresses. Abscisic acid (ABA) and H2O2 played positive roles in the induction of SiNF-YA1 and SiNF-YB8 under stress treatments. Transient luciferase (LUC) expression assays revealed that SiNF-YA1 and SiNF-YB8 could activate the LUC gene driven by the tobacco (Nicotiana tobacam) NtERD10, NtLEA5, NtCAT, NtSOD, or NtPOD promoter under normal or stress conditions. Overexpression of SiNF-YA1 enhanced drought and salt tolerance by activating stress-related genes NtERD10 and NtCAT1 and by maintaining relatively stable relative water content (RWC) and contents of chlorophyll, superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) and malondialdehyde (MDA) in transgenic lines under stresses. SiNF-YB8 regulated expression of NtSOD, NtPOD, NtLEA5, and NtERD10 and conferred relatively high RWC and chlorophyll contents and low MDA content, resulting in drought and osmotic tolerance in transgenic lines under stresses. Therefore, SiNF-YA1 and SiNF-YB8 could activate stress-related genes and improve physiological traits, resulting in tolerance to abiotic stresses in plants. All these results will facilitate functional characterization of foxtail millet NF-Ys in future studies.