Prolonged in vivo functional assessment of the mouse oviduct using optical coherence tomography through a dorsal imaging window.
Prolonged in vivo functional assessment of the mouse oviduct using optical coherence tomography through a dorsal imaging window.
复制标题
使用光学相干断层扫描通过背侧成像窗口对小鼠输卵管进行长期体内功能评估。
DOI：
10.1002/jbio.201700316
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发表时间：
2018
影响因子：
2.8
通讯作者：
Larina,IrinaV
中科院分区：
文献类型：
--
作者：
Wang,Shang;Syed,Riana;Grishina,OlgaA;Larina,IrinaV
输卵管是哺乳动物配子运输、受精和着床前胚胎发育的环境。虽然有越来越多的证据表明不孕症与输卵管功能受损有关，但输卵管在正常和受损的生殖过程中所起的具体作用仍不清楚。小鼠是研究人类生殖的重要哺乳动物模型。然而，目前大多数小鼠输卵管的分析依赖于静态组织学或2D可视化，并且无法提供该器官的动态和体积表征。缺乏成像通路阻碍了对输卵管及其相关生殖事件的纵向实时分析，限制了对受精和植入前妊娠机制方面的理解。为了解决这一限制，我们报告了一种3D成像方法，使小鼠输卵管在体内的长期功能评估。通过将光学相干断层扫描与背侧成像窗口相结合，该方法允许输卵管动态的扩展体积可视化，这在以前是无法实现的。该方法用于输卵管收缩的定量分析、纤毛跳动频率的时空表征和纵向成像。这种新的方法是一个有用的在体内成像平台，在哺乳动物繁殖的各种活的研究。
The oviduct (or fallopian tube) serves as an environment for gamete transport, fertilization and preimplantation embryo development in mammals. Although there has been increasing evidence linking infertility with disrupted oviduct function, the specific roles that the oviduct plays in both normal and impaired reproductive processes remain unclear. The mouse is an important mammalian model to study human reproduction. However, most of the current analyses of the mouse oviduct rely on static histology or 2D visualization, and are unable to provide dynamic and volumetric characterization of this organ. The lack of imaging access prevents longitudinal live analysis of the oviduct and its associated reproductive events, limiting the understanding of mechanistic aspects of fertilization and preimplantation pregnancy. To address this limitation, we report a 3D imaging approach that enables prolonged functional assessment of the mouse oviduct in vivo. By combining optical coherence tomography with a dorsal imaging window, this method allows for extended volumetric visualization of the oviduct dynamics, which was previously not achievable. The approach is used for quantitative analysis of oviduct contraction, spatiotemporal characterization of cilia beat frequency and longitudinal imaging. This new approach is a useful in vivo imaging platform for a variety of live studies in mammalian reproduction.