The InIB protein of Listeria monocytogenes is sufficient to promote entry into mammalian cells
The InIB protein of Listeria monocytogenes is sufficient to promote entry into mammalian cells
复制标题
DOI：
10.1046/j.1365-2958.1998.00750.x
复制
发表时间：
1998-03-01
影响因子：
3.6
通讯作者：
Cossart, P
中科院分区：
文献类型：
--
作者：
Braun, L;Ohayon, H;Cossart, P
InlB是细菌进入哺乳动物细胞所需的两种单核细胞增生李斯特菌侵入蛋白之一，进入人上皮细胞如Caco-2需要InlA，而进入培养的肝细胞和一些上皮或成纤维细胞系如Vero、HEp-2和HeLa细胞需要InlB，InlB介导的进入需要酪氨酸磷酸化，细胞骨架重排和宿主蛋白磷酸肌醇（PI）3-激酶的激活，可能是响应于受体的参与。在这项研究中，我们首次证明InlB足以促进内化。实际上，用InlB包被正常非侵入性细菌或惰性乳胶珠粒导致内化到哺乳动物细胞中。此外，可溶形式的InlB似乎也促进非侵入性细菌的摄取，尽管水平非常低。类似于L的条目。在单核细胞增多症中，InIB包被的珠粒的摄取需要宿主细胞中的酪氨酸磷酸化、PI 3-激酶活性和细胞骨架重组。总之，这些数据表明InlB足以进入L。单核细胞增多症进入宿主细胞，并表明这种蛋白质是宿主细胞信号传导途径的效应物。
InlB is one of the two Listeria monocytogenes invasion proteins required for bacterial entry into mammalian cells, Entry into human epithelial cells such as Caco-2 requires InlA, whereas InlB is needed for entry into cultured hepatocytes and some epithelial or fibroblast cell lines such as Vero, HEp-2 and HeLa cells, InlB-mediated entry requires tyrosine phosphorylation, cytoskeletal rearrangements and activation of the host protein phosphoinositide (PI) 3-kinase, probably in response to engagement of a receptor, In this study, we demonstrate for the first time that InlB is sufficient to promote internalization. Indeed, coating of normally non-invasive bacteria or inert latex beads with InlB leads to internalization into mammalian cells, In addition, a soluble form of InlB also appears to promote uptake of non-invasive bacteria, albeit at a very low level. Similar to entry of L. monocytogenes, uptake of InlB-coated beads required tyrosine phosphorylation in the host cell, PI 3-kinase activity and cytoskeletal reorganization. Taken together, these data indicate that InlB is sufficient for entry of L. monocytogenes into host cells and suggest that this protein is an effector of host cell signalling pathways.