Modulation of Lubricin Biosynthesis and Tissue Surface Properties Following Cartilage Mechanical Injury
Modulation of Lubricin Biosynthesis and Tissue Surface Properties Following Cartilage Mechanical Injury
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DOI：
10.1002/art.24143
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发表时间：
2009-01-01
影响因子：
--
通讯作者：
Flannery, Carl R.
中科院分区：
文献类型：
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作者：
Jones, Aled R. C.;Chen, Shuodan;Flannery, Carl R.
客观的。评估损伤性压迫对关节软骨内不同深度润滑素生物合成的影响，并检查机械损伤后关节面结构和功能的变化。方法。牛软骨外植体分为1级（具有完整的关节面）和2级（含有中深层软骨）。机械损伤后，通过定量逆转录酶-聚合酶链反应监测润滑素信使 RNA (mRNA) 水平，并通过蛋白质印迹和免疫组织化学分析可溶性或软骨相关润滑素蛋白。通过组织学染色评估软骨形态，并通过摩擦测试评估组织功能。结果。损伤两天后，1 级外植体中润滑素 mRNA 表达上调至 3 倍，而 2 级外植体中润滑素 mRNA 表达下调。培养 6 天后，1 级软骨中的润滑素表达恢复到对照水平。类似地，对于 1 级外植体来说，润滑素蛋白的合成和分泌随着损伤而增加，而对于 2 级软骨来说则减少。组织学染色揭示了损伤后 I 级外植体关节表面糖胺聚糖和胶原蛋白含量的变化。受伤的 1 级外植体表现出相对于对照增加的摩擦系数。结论。我们的研究结果表明，润滑素生物合成的增加似乎是表层软骨对损伤性压缩的早期短暂反应。然而，损伤时会发生明显的形态变化，似乎会损害组织的摩擦特性。
Objective. To evaluate the effects of injurious compression on the biosynthesis of lubricin at different depths within articular cartilage and to examine alterations in structure and function of the articular surface following mechanical injury.Methods. Bovine cartilage explants were subdivided into level 1, with intact articular surface, and level 2, containing middle and deep zone cartilage. Following mechanical injury, lubricin messenger RNA (mRNA) levels were monitored by quantitative reverse transcriptase-polymerase chain reaction, and soluble or cartilage-associated lubricin protein was analyzed by Western blotting and immunohistochemistry. Cartilage morphology was assessed by histologic staining, and tissue functionality was assessed by friction testing.Results. Two days after injury, lubricin mRNA expression was up-regulated similar to 3-fold for level 1 explants and was down-regulated for level 2 explants. Lubricin expression in level 1 cartilage returned to control levels after 6 days in culture. Similarly, lubricin protein synthesis and secretion increased in response to injuryfor level 1 explants and decreased for level 2 cartilage. Histologic staining revealed changes in the articular surface of level I explants following injury, with respect to glycosaminoglycan and collagen content. Injured level 1 explants displayed an increased coefficient of friction relative to controls.Conclusion. Our findings indicate that increased lubricin biosynthesis appears to be an early transient response of surface-layer cartilage to injurious compression. However, distinct morphologic changes occur with injury that appear to compromise the frictional properties of the tissue.