Immunity to squamous carcinoma in mice immunized with dendritic cells transfected with genomic DNA from squamous carcinoma cells.
Immunity to squamous carcinoma in mice immunized with dendritic cells transfected with genomic DNA from squamous carcinoma cells.
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用转染鳞状癌细胞基因组 DNA 的树突状细胞免疫小鼠，对鳞状癌具有免疫力。
DOI：
10.1038/sj.cgt.7700847
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发表时间：
2005
影响因子：
6.4
通讯作者：
Cohen,EdwardP
中科院分区：
文献类型：
--
作者：
O-Sullivan,InSug;Ng,LaurenK;Martinez,DonM;Kim,TaeS;Chopra,Amla;Cohen,EdwardP
鳞状细胞癌（SCC）的免疫治疗在疾病的早期阶段增加了成功的可能性。我们报告了一种新的疫苗接种策略，旨在从微克量的肿瘤组织中制备SCC疫苗，从而能够治疗具有微小残留疾病的患者。通过将来自KLN 205细胞（DBA/2小鼠来源的SCC细胞系）的剪切的基因组DNA片段（25 kb）转移到同基因骨髓来源的成熟树突状细胞（DC）中来制备疫苗。超过90%的转染DC从肿瘤中摄取DNA，并且转移的基因以蛋白质形式表达。DC表达成熟DC的特征性的CD 11 c、CD 11b和共刺激分子CD 40、CD 80和CD 86。对KLN 205细胞生长高度敏感的同基因DBA/2 J小鼠静脉内（iv）注射转染的DC，随后皮下（sc）注射肿瘤细胞。结果表明，转染细胞具有较强的免疫原性，能延长荷瘤小鼠的生存时间（P<0.05）。001），相对于各对照组中的小鼠。酶联免疫斑点法（ELISPOT IFN-γ）检测显示转染DC免疫的小鼠中细胞介导的针对SCC的免疫被激活。两个独立的体外细胞毒性试验表明，在用转染细胞免疫的小鼠中存在针对SCC的稳健的细胞介导的免疫。
Immunotherapy of squamous cell carcinoma (SCC) at an early stage of the disease increases the likelihood of success. We report a new vaccination strategy designed to prepare SCC vaccines from microgram amounts of tumor tissue, enabling the treatment of patients with minimal residual disease. The vaccine was prepared by transfer of sheared genomic DNA-fragments (25 kb) from KLN205 cells, an SCC cell line of DBA/2 mouse origin, into syngeneic bone marrow-derived mature dendritic cells (DCs). More than 90% of the transfected DCs took up DNA from the neoplasm and transferred genes were expressed as protein. The DCs expressed CD11c, CD11b, and the costimulatory molecules CD40, CD80 and CD86, characteristic of mature DCs. Syngeneic DBA/2J mice, highly susceptible to the growth of KLN205 cells, were injected intravenously (iv) with the transfected DCs, followed by a subcutaneous (sc) injection of the tumor cells. The strong immunogenic properties of the transfected cells were indicated by the finding that the survival of the tumor-bearing mice was prolonged (P<. 001), relative to that of mice in various control groups. Enzyme-linked immuno spot (ELISPOT IFN-γ) assays revealed the activation of cell-mediated immunity directed toward the SCC in mice immunized with the transfected DCs. Two independent in vitro cytotoxicity assays indicated the presence of robust cell-mediated immunity directed toward the SCC in mice immunized with the transfected cells.
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DOI：
10.1111/j.1432-1033.1986.tb09925.x
发表时间：
1986
期刊：
European journal of biochemistry
影响因子：
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作者：
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DOI：
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发表时间：
1988
期刊：
European journal of biochemistry
影响因子：
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影响因子：
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作者：
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DOI：
--
发表时间：
1980
期刊：
European Journal of Biochemistry
影响因子：
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作者：
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通讯作者：
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