The exonuclease ERI-1 has a conserved dual role in 5.8S rRNA processing and RNAi
The exonuclease ERI-1 has a conserved dual role in 5.8S rRNA processing and RNAi
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DOI：
10.1038/nsmb.1411
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发表时间：
2008-05-01
影响因子：
16.8
通讯作者：
Ruvkun, Gary
中科院分区：
文献类型：
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作者：
Gabel, Harrison W.;Ruvkun, Gary
外切酶ERI-1负向调控秀丽隐杆线虫和pombe Schizosaccharomyces的RNA干扰，是秀丽隐杆线虫产生内源性小干扰RNA所必需的。我们发现，在秀丽隐杆线虫和pombe线虫中，ERI-1都执行5.8S核糖体RNA的3'端加工。在秀丽隐杆线虫中，ERI-1的两种蛋白异构体定位于细胞质，在核糖体RNA加工和RNA干扰的负调控中各有不同的功能。
The exonuclease ERI-1 negatively regulates RNA interference in Caenorhabditis elegans and Schizosaccharomyces pombe, and is required for production of some C. elegans endogenous small interfering RNAs. We show that ERI-1 performs 3' end processing of the 5.8S ribosomal RNA in both C. elegans and S. pombe. In C. elegans, two protein isoforms of ERI-1 are localized to the cytoplasm, and each has distinct functions in ribosomal RNA processing and negative regulation of RNA interference.