UV-B protective effect of a polyacylated anthocyanin, HBA, in flower petals of the blue morning glory, Ipomoea tricolor cv. Heavenly Blue
UV-B protective effect of a polyacylated anthocyanin, HBA, in flower petals of the blue morning glory, Ipomoea tricolor cv. Heavenly Blue
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DOI：
10.1016/j.bmc.2005.01.011
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发表时间：
2005-03-15
影响因子：
3.5
通讯作者：
Kondo, T
中科院分区：
文献类型：
--
作者：
Mori, M;Yoshida, K;Kondo, T
研究了聚酰化花色苷(HBA)对牵牛花(Ipomoea tricolor cv.)天蓝)对UV-B诱导的DNA损伤的拮抗作用。我们首先明确了HBA在表皮液泡中的浓度为12 mM，然后构建了一台类似花瓣组织的UV-B照射装置，以评估HBA的筛选效果。HBA滤膜可将单色(280 nm和310 nm)或宽幅UV-B诱导的DNA损伤完全减少到与活体花瓣表皮相同的分子数。然而，稀释的HBA溶液和三乙酰HBA没有相同的还原效果。HBA比三酰化HBA对太阳辐射的耐受性更强。这些数据有力地表明，花瓣中的聚酰化花青素可以有效地屏蔽有害的UV-B。这种作用很大程度上可能是由于芳香族酰基残基所致。(C)2005爱思唯尔有限公司。保留所有权利。
The protective effects of polyacylated anthocyanin, heavenly blue anthocyanin (HBA), in blue flower petals of morning glory (Ipomoea tricolor cv. Heavenly Blue) against UV-B induced DNA damage were examined. We first clarified the concentration of HBA in epidermal vacuoles to be 12 mM, and then constructed a UV-B irradiating apparatus resembling flower petal tissue to assess the screening effect of HBA. Monochromatic (280 and 310 nm) or broad UV-B induced DNA lesions were reduced completely by the HBA filter to the same molecular numbers as those in living petal epidermis. However, diluted HBA solution and trisdeacyl HBA did not have the same reduction effect. HBA was more tolerant to solar radiation than trisdeacyl HBA. These data strongly suggest that polyacylated anthocyanins in flower petals can screen harmful UV-B efficiently. This action might be largely due to aromatic acyl residues. (c) 2005 Elsevier Ltd. All rights reserved.