Microsatellite Markers for Bearded Capuchins (Sapajus libidinosus): Transferability and Characterization
Microsatellite Markers for Bearded Capuchins (Sapajus libidinosus): Transferability and Characterization
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DOI：
10.1590/0001-3765202120190802
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发表时间：
2021-01-01
期刊：
影响因子：
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通讯作者：
BEZERRA, BRUNA
中科院分区：
文献类型：
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作者：
MORAES, BÁRBARA L.C. DE;BORGES, DANIELA B.;BEZERRA, BRUNA
由于破碎化、栖息地丧失和非法宠物交易，天然沙蜂种群数量持续下降。他们生活在卡廷加灌丛森林中，那里已经失去了50%以上的原始植被。由于缺乏对libidinosus种群遗传学的研究，我们不知道它们是如何受到这种惊人的栖息地丧失和其他人为干扰的影响。目前还没有多态标记可用于研究利比多诺斯的多样性和群体遗传学。因此，我们的目的是测试14个微卫星标记的可移植性。这些微卫星先前从与研究物种属于同一亚科的白面卷尾猴（Cebus capucinus）中分离得到。我们发现6个测试的微卫星标记（四核苷酸）在我们的目标物种中被交叉扩增。所有的位点都是多态的。等位基因数为4 ~ 7个，期望杂合度为0.588 ~ 0.869。本研究中转移到s.l libidinosus的微卫星标记将成为评估野生种群和圈养种群遗传变异性的有价值的工具。它们将大大降低微卫星隔离的成本，有助于优先考虑巴西目前有限的研究和保护预算。
Natural Sapajus libidinosus populations are in continuous decline due to fragmentation, habitat loss, and the illegal pet trade. They live in Caatinga scrub forests, which already lost over 50% of their original cover. The lack of studies on S. libidinosus population genetics means that we do not know how they are being affected by this striking habitat loss and other anthropogenic disturbances. Polymorphic markers are not available for the study of S. libidinosus diversity and population genetics. Thus, here we aimed to test the transferability of 14 microsatellite markers to S. libidinosus. These microsatellites were previously isolated from Cebus capucinus (white-faced capuchin), species belonging to the same subfamily (Cebinae) as the study species. We found that six of the tested microsatellite markers (tetra-nucleotide) were cross-amplified in our target species. All loci were polymorphic. The number of alleles varied from 4 to 7, and the expected heterozygosity ranged from 0.588 to 0.869. The microsatellite markers transferred to S. libidinosus and characterised in our study will be valuable tools to evaluate the genetic variability of both wild and captive populations. They will considerably reduce the costs of microsatellite isolations, helping to prioritise currently limited research and conservation budgets in Brazil.