Boronate affinity solid-phase extraction based on functionalized magnesia–zirconia composite for enrichment of nucleosides in human urine
Boronate affinity solid-phase extraction based on functionalized magnesia–zirconia composite for enrichment of nucleosides in human urine
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DOI：
10.1039/c2ay26420j
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发表时间：
2013-03
影响因子：
3.1
通讯作者：
Haibo He;Yanxiang Sun;Bin Li;Qiongwei Yu;Tianlin Wang;Yuqi Feng
中科院分区：
文献类型：
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作者：
Haibo He;Yanxiang Sun;Bin Li;Qiongwei Yu;Tianlin Wang;Yuqi Feng
通过路易斯酸碱化学吸附很容易合成了一种新型硼酸功能化氧化镁-氧化锆（ZM）基材料。元素分析、电感耦合等离子体原子发射光谱仪（ICP-AES）和傅里叶变换红外光谱仪（FT-IR）证明3-氨基苯硼酸衍生物成功附着在ZM表面上。在本工作中，通过使用所得材料作为固相萃取 4 种核苷（即胞苷、尿苷、鸟嘌呤和腺苷）的吸附剂来评估所得材料对顺式二醇结合的亲和力。结果表明，这种硼酸改性氧化镁-氧化锆复合微粒在宽pH范围和高盐浓度的体系中具有优异的捕获核苷的性能，并且使用TFA-ACN-H2O（5：50：45，v/v/v）溶液作为洗脱剂可以有效地释放核苷。添加到未经处理的尿液样本中（即未稀释或 pH 调整）的 4 种核苷显着富集，回收率在 86-109% 范围内，LOD 值在 0.003-0.010 μg mL−1 范围内。构建的校准曲线范围为0.05至2.0 μg mL−1，显示出良好的线性相关系数（R ≥ 0.9961）和精密度（RSD < 9%）。所提出的材料将允许采用替代提取策略来识别和定量分析生物样品中的顺式二醇结构化合物。
A novel boronic acid functionalized magnesia–zirconia (ZM) based material was facile synthesized by Lewis acid–base chemisorption. The elemental analysis, inductively coupled plasma atomic emission spectrometer (ICP-AES) and Fourier transform infrared spectrometer (FT-IR) have proven the successful attachment of 3-aminophenylboronic acid derivative onto ZM surface. In the present work, the affinity of the resulting material towards cis-diols binding was evaluated by using it as the sorbent for solid-phase extraction of 4 nucleosides (namely cytidine, uridine, guanine and adenosine). The results show that such boronic acid modified magnesia–zirconia composite microparticles were excellent for capturing nucleosides in the system with a wide range of pH and high salt concentration, which could be effectively released using TFA–ACN–H2O (5 : 50 : 45, v/v/v) solution as elution. The 4 nucleosides spiked to untreated urine sample (i.e., no diluting or pH adjustment) were prominently enriched with recoveries in the range of 86–109% and LOD values in the range of 0.003–0.010 μg mL−1. The constructed calibration curves range from 0.05 to 2.0 μg mL−1 showing good linearity correlation coefficient (R ≥ 0.9961) and precision (RSD < 9%). The proposed material would allow for an alternative extraction strategy for identification and quantitative analysis of cis-diols structure compounds in biological samples.