Synthetic Cis-Jasmone Exposure Induces Wheat and Barley Volatiles that Repel the Pest Cereal Leaf Beetle, Oulema melanopus L.
Synthetic Cis-Jasmone Exposure Induces Wheat and Barley Volatiles that Repel the Pest Cereal Leaf Beetle, Oulema melanopus L.
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DOI：
10.1007/s10886-013-0281-4
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发表时间：
2013-05-01
影响因子：
2.3
通讯作者：
Piesik, Dariusz
中科院分区：
文献类型：
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作者：
Delaney, Kevin J.;Wawrzyniak, Maria;Piesik, Dariusz
植物化学信息素顺式茉莉酮引发/诱导植物抗性，阻止食草动物并吸引天敌。我们研究了植物暴露于三种合成的顺式茉莉酮剂量（50 μ l的1、100和1 × 10（4）ng μ l（-1））和暴露时间（1、3和6 h）后，对冬小麦和春大麦中挥发性有机化合物（VOCs）的诱导作用。在Y形管嗅觉仪中检查了谷叶甲Oulema melanopus成虫对顺式茉莉酮诱导的植物VOC花束和两种VOC合成混合物（3种绿色叶挥发物（GLV）; 4种萜烯+吲哚）的行为反应。在两种谷物中，在暴露于顺式茉莉酮后，八种VOC [（Z）-3-己醛、（Z）-3-己醇、（Z）-3-乙酸己酯、（Z）-β-罗勒烯、芳樟醇、β-香芹烯、（E）-α-法呢烯和吲哚]被诱导100至1000倍。两种谷物中的诱导程度通常与暴露剂量和持续时间的增加呈正线性相关。然而，挥发性有机化合物的排放率仅为相似的2倍大，从植物暴露于最高与最低的顺式茉莉酮暴露剂量（1 Au 10（4）的差异）或持续时间（6倍的差异）。雌雄O.在植物暴露于高顺式茉莉酮剂量（1 Au 10（4）ng mul（-1））后，两种谷物VOC花束都阻止了黑尾果蝇，而在植物暴露于低剂量（1 ng mul（-1））后，雌性果蝇也被阻止，但被未暴露的植物VOC花束吸引。两种O.在两种浓度（25 ng中心点min（-1）; 125 ng中心点min（-1））下，萜烯/吲哚和GLV混合物排斥黑足鼠，但吸引最低剂量（1 ng中心点min（-1））的GLV混合物。生物学相关的低剂量茉莉酮可能具有诱导大田作物VOCs的生态活性和潜力，以阻止O。黑星。
The plant semiochemical cis-jasmone primes/induces plant resistance that deters herbivores and attracts natural enemies. We studied the induction of volatile organic compounds (VOCs) in winter wheat and spring barley after exposure of plants to three synthetic cis-jasmone doses (50 mu l of 1, 100, and 1 x 10(4) ng mu l(-1)) and durations of exposure (1, 3, and 6 h). Cereal leaf beetle, Oulema melanopus, adult behavioral responses were examined in a Y-tube olfactometer to cis-jasmone induced plant VOC bouquets and to two synthetic blends of VOCs (3 green leaf volatiles (GLVs); 4 terpenes + indole). In both cereals, eight VOCs [(Z)-3-hexanal, (Z)-3-hexanol, (Z)-3-hexanyl acetate, (Z)-beta-ocimene, linalool, beta-caryophyllene, (E)-aEuro"farnesene, and indole] were induced 100- to 1000-fold after cis-jasmone exposure. The degree of induction in both cereals was usually positively and linearly associated with increasing exposure dose and duration. However, VOC emission rate was only similar to 2-fold greater from plants exposed to the highest vs. lowest cis-jasmone exposure doses (1 Au 10(4) difference) or durations (6-fold difference). Male and female O. melanopus were deterred by both cereal VOC bouquets after plant exposure to the high cis-jasmone dose (1 Au 10(4) ng mu l(-1)), while females were also deterred after plant exposure to the low dose (1 ng mu l(-1)) but attracted to unexposed plant VOC bouquets. Both O. melanopus sexes were repelled by terpene/indole and GLV blends at two concentrations (25 ng center dot min(-1); 125 ng center dot min(-1)), but attracted to the lowest dose (1 ng center dot min(-1)) of a GLV blend. It is possible that the biologically relevant low cis-jasmone dose has ecological activity and potential for inducing field crop VOCs to deter O. melanopus.