Twist expression associated with the epithelial-mesenchymal transition in gastric cancer
Twist expression associated with the epithelial-mesenchymal transition in gastric cancer
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Twist表达与胃癌上皮间质转化相关
DOI：
10.1007/s11010-012-1333-8
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发表时间：
2012-08-01
影响因子：
4.3
通讯作者：
Hang, Xiao-sheng
中科院分区：
文献类型：
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作者：
Liu, Ai-ning;Zhu, Zhi-Hua;Hang, Xiao-sheng
本研究旨在探讨Twist在胃癌组织中的表达及其与上皮间质转化(EMT)的相关性。采用RT-PCR和Western blot方法检测61例胃癌组织及癌旁正常组织中Twist、E-cadherin、Vimentin mRNA和蛋白的表达情况。胃癌组织中Twist、E-cadherin、Vimentin mRNA表达阳性率为73.9。分别为 40.6 和 60.9%；与邻近正常组织中这些基因的表达（2.9、75.4 和 27.5%）相比，差异显着（p<0.05）。胃癌组织中E-cadherin蛋白表达水平显着低于癌旁正常组织（p<0.05）。 MKN45细胞转染Twist siRNA后，Twist蛋白表达显着降低（p<0.05），E-cadherin蛋白表达显着升高，通过Transwell小室的细胞数显着低于未转染对照组和转染对照组（p<0.05）。 Twist可能与胃癌的上皮间质转化以及胃癌的发生、侵袭和转移有关。
This study aimed to investigate the expression of Twist in gastric cancer tissues and its correlation between Twist and the epithelial-mesenchymal transition (EMT). By means of RT-PCR and Western blot, the mRNA and protein expressions of Twist, E-cadherin, and Vimentin in 61 gastric cancer tissues and adjacent normal tissues were detected. The positive rates of Twist, E-cadherin, and Vimentin mRNA expression in gastric cancer tissues were 73.9. 40.6, and 60.9 %, respectively; compared to the expression of these genes in adjacent normal tissues (2.9, 75.4, and 27.5 %), the differences were significant (p< 0.05). The E-cadherin protein expression level in gastric cancer tissues was significantly lower than that in the adjacent normal tissues (p< 0.05). After the transfection of Twist siRNA into the MKN45 cells, the protein expression of Twist was significantly reduced (p< 0.05), the protein expression of E-cadherin was significantly increased, and the number of cells that passed through the Transwell chamber was significantly lower than that in the non-transfected control group as well as the transfected control group (p< 0.05). Twist may be associated with the epithelial-mesenchymal transition in gastric cancer and the tumorigenesis, invasion, and metastasis of gastric cancer.