Continuous proliferation of murine antigen-specific helper T lymphocytes in culture.
Continuous proliferation of murine antigen-specific helper T lymphocytes in culture.
复制标题
鼠抗原特异性辅助 T 淋巴细胞在培养物中持续增殖。
DOI：
10.1084/jem.150.6.1510
复制
发表时间：
1979-12-01
影响因子：
15.3
通讯作者：
Watson, J
中科院分区：
文献类型：
--
作者：
Watson, J
小鼠辅助性T细胞在体内被羊或马红细胞抗原激活，已在培养中作为连续细胞系建立。T细胞需要T细胞生长因子（TCGF）的存在才能持续增殖。从小鼠、大鼠或人体内纯化的TCGF都能刺激小鼠t细胞的生长。在连续的t细胞系中，t细胞有丝分裂原豆蛋白A和植物血凝素不刺激细胞增殖。所有在TCGF存在下生长的细胞都表达Thy-1抗原。t细胞系的辅助活性既具有抗原特异性，又对同源或F1 B细胞有效。t细胞系的上代不含抗原特异性或非特异性辅助因子。虽然一些t细胞系在培养中表现出稳定的辅助活性超过50周，但其他细胞系表现出效应功能的逐渐下降。用于培养和维持T细胞增殖的程序应该适合于从每个亚类中选择和生长抗原特异性效应T细胞。
Murine helper T cells activated to sheep or horse erythrocyte antigens in vivo have been established as continuous cell lines in culture. T cells require the presence of a T-cell growth factor (TCGF) for continuous proliferation. TCGF purified from murine, rat, or human sources all stimulate murine T-cell growth. The T-cell mitogens concanavalin A and phytohemagglutinin do not stimulate cell proliferation in continuous T-cell lines. All cells that grow in the presence of TCGF express Thy-1 antigens. Helper activity of T-cell lines is both antigen specific and effective for syngeneic or F1 B cells. Supernates from T-cell lines do not contain antigen-specific or nonspecific helper factors. Although several T-cell lines have shown stable helper activity for greater than 50 wk in culture, other cell lines have shown a gradual decline in effector function. The procedure used to establish and maintain proliferation of T cells in culture should be suitable for the selection and growth of antigen-specific effector T cells from each subclass.