Visual response properties of burst and tonic firing in the mouse dorsal lateral geniculate nucleus
Visual response properties of burst and tonic firing in the mouse dorsal lateral geniculate nucleus
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DOI：
10.1152/jn.00445.2004
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发表时间：
2005-06-01
影响因子：
2.5
通讯作者：
Thompson, ID
中科院分区：
文献类型：
--
作者：
Grubb, MS;Thompson, ID
丘脑中继细胞以两种模式激发动作电位：爆发和紧张。以往的研究表明，这两种模式与背外侧膝状体核（dLGN）的棘波所携带的视觉信息的显着差异有关。在这里，我们描述的视觉反应特性的突发和紧张性放电的小鼠dLGN。在氟烷和一氧化二氮麻醉下，在体内进行单个膝状体细胞的细胞外活动记录。在确认用于从这些记录中分离突发尖峰的标准识别具有在其他物种和制剂中描述的突发发射的属性的发射事件后，我们表明在视觉刺激期间发生在小鼠dLGN中的突发发射。然后，我们比较了突发和紧张性放电在广泛的视觉反应特征。虽然这两种发射模式在空间求和或空间频率调谐方面没有不同，但它们在时间域中显示出显着差异。爆发棘波相对于其主音对应物是相位提前的。爆发性放电也更整流，具有更尖锐的时间频率调谐，并且比紧张性放电更喜欢较低的时间频率。此外，对比度-响应曲线对于爆发响应更像台阶。最后，我们提出的分析，描述的刺激检测能力和尖峰定时的可靠性，突发和紧张性放电。
Thalamic relay cells fire action potentials in two modes: burst and tonic. Previous studies in cats have shown that these two modes are associated with significant differences in the visual information carried by spikes in the dorsal lateral geniculate nucleus (dLGN). Here we describe the visual response properties of burst and tonic firing in the mouse dLGN. Extracellular recordings of activity in single geniculate cells were performed under halothane and nitrous oxide anesthesia in vivo. After confirming that the criteria used to isolate burst spikes from these recordings identify firing events with properties described for burst firing in other species and preparations, we show that burst firing in the mouse dLGN occurs during visual stimulation. We then compare burst and tonic firing across a wide range of visual response characteristics. While the two firing modes do not differ with respect to spatial summation or spatial frequency tuning, they show significant differences in the temporal domain. Burst spikes are phase advanced relative to their tonic counterparts. Burst firing is also more rectified, possesses sharper temporal frequency tuning, and prefers lower temporal frequencies than tonic firing. In addition, contrast-response curves are more step-like for burst responses. Finally, we present analyses that describe the stimulus detection abilities and spike timing reliability of burst and tonic firing.