End product control of tryptophan biosynthesis in extracts and intact cells of the higher plant Nicotiana tabacum var. Wisconsin 38.
End product control of tryptophan biosynthesis in extracts and intact cells of the higher plant Nicotiana tabacum var. Wisconsin 38.
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高等植物烟草提取物和完整细胞中色氨酸生物合成的最终产物控制。
DOI：
10.1016/0304-4165(71)90023-7
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发表时间：
1971
期刊：
影响因子：
--
通讯作者：
Stanley E. Mills
中科院分区：
文献类型：
--
作者：
W. Belser;J. Murphy;D. P. Delmar;Stanley E. Mills
研究了利用终产物抑制法对高等植物烟草培养细胞色氨酸合成途径的控制。威斯康星州38.烟草碱催化氯酸转化为邻氨基苯甲酸，第一步独特的甲苯-色氨酸形式的氯酸转化为邻氨基苯甲酸，第一步独特的甲苯-色氨酸生物合成。酶的催化活性可被热破坏，当缺少谷氨酰胺或Mg ~（2+）时不发生反应，在细胞提取液中，此催化活性被色氨酸抑制。在完整细胞的实验中，高色氨酸池的存在减少了放射性碳从前体莽草酸流入生物合成L-色氨酸的流量。所得数据与体内终产物抑制的操作一致
A study has been made of the control by end-product inhibition of the tryptophan biosynthetic pathway in cultured cells of the higher plant,Nicotiana tabacum, var. Wisconsin 38.Crude extracts of callus tissue obtained fromN. tabacumcatalyzed the conversion of chorismic acid to anthranilic acid, the first step unique tol-tryptophan version of chorismic acid to anthranilic acid, the first step unique tol-tryptophan biosynthesis. The catalytic activity was destroyed by heat and no reaction occured with the ommission of either glutamine of Mg2+.In cell extracts this catalytic activity was inhibited byl-tryptophan. The inhibition was competitive with chorismate.In experiments with intact cells the presence of highl-tryptophan pools reduced the flow of radioactive carbon from the precursor, shikimic acid, into biosyntheticL-tryptophan. The data obtained were consistent with the operation of end-product inhibitionin vivo