50 Hz extremely low frequency electromagnetic fields enhance protein carbonyl groups content in cancer cells: effects on proteasomal systems.
50 Hz extremely low frequency electromagnetic fields enhance protein carbonyl groups content in cancer cells: effects on proteasomal systems.
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DOI：
10.1155/2009/834239
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发表时间：
2009
影响因子：
--
通讯作者：
Fioretti E
中科院分区：
文献类型：
--
作者：
Eleuteri AM;Amici M;Bonfili L;Cecarini V;Cuccioloni M;Grimaldi S;Giuliani L;Angeletti M;Fioretti E
电磁场是延长自由基寿命的一个被评估的原因。本研究旨在研究极低频电磁场对蛋白质氧化的影响，以及对氧化蛋白质降解的复合体--20S蛋白酶体功能的影响。将CaCO2细胞暴露于1mT、50赫兹的电磁场中24-72小时。该处理诱导了细胞生长和蛋白质氧化的时间依赖性增加，在TPA存在时更加明显，而细胞存活率没有检测到变化。将细胞暴露在50赫兹的电磁场中，导致20S蛋白酶体催化成分的全球激活，特别是在暴露72小时和TPA存在的情况下。发现EGCG，一种天然的抗氧化剂化合物，抵消了与场相关的促氧化作用，这表明蛋白酶体活性的增加是由于细胞内自由基的增强。
Electromagnetic fields are an assessed cause of prolonging free radicals lifespan. This study was carried out to investigate the influence of extremely low frequency electromagnetic fields on protein oxidation and on the 20S proteasome functionality, the complex responsible for the degradation of oxidized proteins. Caco 2 cells were exposed, for 24–72 hours, to 1 mT, 50 Hz electromagnetic fields. The treatment induced a time-dependent increase both in cell growth and in protein oxidation, more evident in the presence of TPA, while no changes in cell viability were detected. Exposing the cells to 50 Hz electromagnetic fields caused a global activation of the 20S proteasome catalytic components, particularly evident at 72 hours exposure and in the presence of TPA. The finding that EGCG, a natural antioxidant compound, counteracted the field-related pro-oxidant effects demonstrates that the increased proteasome activity was due to an enhancement in intracellular free radicals.
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影响因子：
4.8
作者：
Eleuteri, AM;Kohanski, RA;Orlowski, M
通讯作者：
Orlowski, M
DOI：
10.1073/pnas.0702489104
发表时间：
2007-07-24
影响因子：
11.1
作者：
Li, Chi;Wang, Xiaoli;White, Carl
通讯作者：
White, Carl
影响因子：
2
作者：
Lee, Sun-Joo;Lee, Kwang-Won
通讯作者：
Lee, Kwang-Won
DOI：
10.1016/s0006-291x(03)00809-x
发表时间：
2003-06-06
影响因子：
3.1
作者：
Grune, T;Merker, K;Davies, KJA
通讯作者：
Davies, KJA
影响因子：
3.9
作者：
Basbous, Jihane;Jariel-Encontre, Isabelle;Piechaczyk, Marc
通讯作者：
Piechaczyk, Marc