Time of the day prioritizes the pool of translating mRNAs in response to heat stress
Time of the day prioritizes the pool of translating mRNAs in response to heat stress
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DOI：
10.1093/plcell/koab113
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发表时间：
2021-04-19
期刊：
影响因子：
11.6
通讯作者：
Nagel, Dawn H.
中科院分区：
文献类型：
--
作者：
Bonnot, Titouan;Nagel, Dawn H.
生物钟帮助生物体预测和协调基因对环境刺激变化的调节反应。在生长限制温度下，一天中的时间调节聚腺苷酸化mRNA的积累。为了应对热胁迫，植物会保存能量并选择性地翻译mRNA。生物钟和/或一天中的时间如何调节核糖体结合的聚腺苷酸化mRNA以响应热应激尚不清楚。对拟南芥翻译mRNA的深入分析发现，一天中的时间门控了大约三分之一的昼夜节律调节的热响应翻译组的反应。具体来说，一天中的时间和热应激相互作用，以优先考虑要翻译的提示中的mRNA库。对于mRNA的子集，我们在白天观察到更强的门控反应，并且优先在表达峰值之前。我们提出了以前被忽视的转录因子（TF）作为调控节点，并表明时钟在选择TF的温度响应中起着重要作用。当压力被删除，重新定义的优先级翻译恢复1小时内，虽然恢复较慢，观察非生物胁迫调节。通过时钟基因和优先TF之间的分层网络连接，我们的工作提供了一个框架，以针对全天热应激耐受性的关键节点。
The circadian clock helps organisms to anticipate and coordinate gene regulatory responses to changes in environmental stimuli. Under growth limiting temperatures, the time of the day modulates the accumulation of polyadenylated mRNAs. In response to heat stress, plants will conserve energy and selectively translate mRNAs. How the clock and/or the time of the day regulates polyadenylated mRNAs bound by ribosomes in response to heat stress is unknown. In-depth analysis of Arabidopsis thaliana translating mRNAs found that the time of the day gates the response of approximately one-third of the circadian-regulated heat-responsive translatome. Specifically, the time of the day and heat stress interact to prioritize the pool of mRNAs in cue to be translated. For a subset of mRNAs, we observed a stronger gated response during the day, and preferentially before the peak of expression. We propose previously overlooked transcription factors (TFs) as regulatory nodes and show that the clock plays a role in the temperature response for select TFs. When the stress was removed, the redefined priorities for translation recovered within 1 h, though slower recovery was observed for abiotic stress regulators. Through hierarchical network connections between clock genes and prioritized TFs, our work provides a framework to target key nodes underlying heat stress tolerance throughout the day.