Identification of the fourth member of the mammalian endoprotease family homologous to the yeast Kex2 protease. Its testis-specific expression.
Identification of the fourth member of the mammalian endoprotease family homologous to the yeast Kex2 protease. Its testis-specific expression.
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DOI：
10.1016/s0021-9258(18)42638-5
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发表时间：
1992-03
期刊：
影响因子：
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通讯作者：
Kazuhisa NakayamaSBT;Won-Sin KimII;S. Torii;Masahiro HosakaS;Tsutomu NakagawaII;Jo IkemizuII;Tadashi Baball;Kazuo MurakamiII
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文献类型：
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作者：
Kazuhisa NakayamaSBT;Won-Sin KimII;S. Torii;Masahiro HosakaS;Tsutomu NakagawaII;Jo IkemizuII;Tadashi Baball;Kazuo MurakamiII
我们使用聚合酶链反应鉴定了一个小鼠睾丸cDNA，该cDNA代表了一个正在增长的哺乳动物内源性蛋白酶的另一个成员，该内源性蛋白酶参与前体蛋白的加工。该cDNA编码了一个655残基蛋白，命名为PC4，包含一个细菌枯草杆菌样催化结构域，与最近表征的前体加工内源性蛋白酶、furin、PC1/PC3、PC2和Kex2密切相关。在该结构域内，PC4的氨基酸序列分别与小鼠furin、小鼠PC1/PC3、小鼠PC2和酵母Kex2的氨基酸序列相同，分别为70、58、55和45%。Northern blot分析显示，PC4 mRNA仅在出生后第20天的睾丸中检测到。该信息主要在圆形精子中表达。这些数据表明，PC4是睾丸生殖细胞中前体加工内蛋白酶的主要候选者，其基因表达在精子发生过程中受到调节。
We used the polymerase chain reaction to identify a mouse testis cDNA that represented another member of a growing class of mammalian endoproteases involved in the processing of precursor proteins. This cDNA encoded a 655-residue protein, designated PC4, containing a bacterial subtilisin-like catalytic domain closely related to those of the recently characterized precursor-processing endoproteases, furin, PC1/PC3, PC2, and Kex2. Within this domain, the amino acid sequence of PC4 was 70, 58, 55, and 45% identical with those of mouse furin, mouse PC1/PC3, mouse PC2, and yeast Kex2, respectively. Northern blot analysis indicated that the PC4 mRNA was detectable only in the testes after the 20th day of postnatal development. Moreover, this message was mainly expressed in the round spermatids. These data suggest that PC4 represents a prime candidate for a precursor-processing endoprotease in the testicular germ cells and that its gene expression is regulated during spermatogenesis.