DNA barcodes for biosecurity: invasive species identification
DNA barcodes for biosecurity: invasive species identification
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DOI：
10.1098/rstb.2005.1713
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发表时间：
2005-10-29
影响因子：
6.3
通讯作者：
Ball, SL
中科院分区：
文献类型：
--
作者：
Armstrong, KF;Ball, SL
生物安全包括防止“生物危害”带来的任何风险，尤其是害虫传播造成的经济影响。分子诊断工具为快速、准确地识别形态不明确的外来物种提供了宝贵的支持。然而，这些工具目前缺乏标准化。它们不利于多个部门或国家的适应，也不利于应对不断变化的虫害优先事项。这里提供的数据表明 DNA 条形码是解决这个问题的一个非常有前途的机会。使用 cox1 序列条形码方法对过去十年在新西兰边境截获的草蛾和果蝇样本的 DNA 进行了重新分析。将物种鉴定与核 rDNA PCR-RFLP 获得的历史数据集进行比较。这些物种的方法之间的一致性分别为 90% 和 96%。改进包括将先前的草丛蛾“未知数”归入科、属或种，并在果蝇物种复合体中进一步解析。分析强调了 DNA 条形码的几个优点，特别是它们的适应性和预测价值。这种方法是一个现实的平台，可以在其上构建更加灵活的系统，有可能在全球范围内采用，以快速准确地识别外来入侵物种。
Biosecurity encompasses protecting against any risk through 'biological harm', not least being the economic impact from the spread of pest insects. Molecular diagnostic tools provide valuable support for the rapid and accurate identification of morphologically indistinct alien species. However, these tools currently lack standardization. They are not conducive to adaptation by multiple sectors or countries, or to coping with changing pest priorities. The data presented here identifies DNA barcodes as a very promising opportunity to address this. DNA of tussock moth and fruit fly specimens intercepted at the New Zealand border over the last decade were reanalysed using the cox1 sequence barcode approach. Species identifications were compared with the historical dataset obtained by PCR-RFLP of nuclear rDNA. There was 90 and 96% agreement between the methods for these species, respectively. Improvements included previous tussock moth 'unknowns' being placed to family, genera or species and further resolution within fruit fly species complexes. The analyses highlight several advantages of DNA barcodes, especially their adaptability and predictive value. This approach is a realistic platform on which to build a much more flexible system, with the potential to be adopted globally for the rapid and accurate identification of invasive alien species.