A layered tungsten disulfide/acetylene black composite based DNA biosensing platform coupled with hybridization chain reaction for signal amplification
A layered tungsten disulfide/acetylene black composite based DNA biosensing platform coupled with hybridization chain reaction for signal amplification
复制标题
基于层状二硫化钨/乙炔黑复合材料的DNA生物传感平台结合杂交链式反应进行信号放大
DOI：
10.1039/c5tb02214b
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发表时间：
2016
影响因子：
7
通讯作者：
陈颖旭
中科院分区：
文献类型：
--
作者：
帅洪磊;黄克靖;陈颖旭
采用简单的水热法制备了二维硫化钨-乙炔黑（WS 2-AB）复合材料，并将其作为DNA生物传感器。将Au纳米粒子（AuNPs）与WS 2-AB复合修饰电极制备成生物传感器，并将其与捕获探针通过Au-S键偶联，再与靶DNA、辅助DNA和bio-H1-bio-H2（H1-H2）进行修饰，进行杂交链反应放大信号。在此，两个DNA发夹H1和H2被识别探针打开。利用杂交链反应产生的切口双螺旋，通过生物素-亲和素反应修饰辣根过氧化物酶，通过过氧化氢+对苯二酚体系的催化反应，产生对靶DNA的信号放大检测。在最佳条件下，所制备的生物传感器在0.001 ~ 100 pM范围内，电流值与靶DNA浓度的对数呈良好的线性关系，检测限低至0.12 fM。该传感器对单碱基错配的DNA序列具有良好的选择性。这项工作将为基于层状WS 2-AB和杂交链反应的其他生物识别事件和基因相关疾病的超灵敏检测开辟一条途径。
A 2-dimensional tungsten disulfide–acetylene black (WS2–AB) composite is synthesized by a simple hydrothermal method to achieve excellent electrochemical properties for applications as a DNA biosensor. The biosensor is fabricated based on the Au nanoparticles (AuNPs) and WS2–AB composite modified electrode, which subsequently is used to couple with a capture probe by an Au–S bond, then modified with target DNA, auxiliary DNA and bio-H1–bio-H2 (H1–H2) to perform hybridization chain reaction for signal amplification. Herein, two DNA hairpins H1 and H2 are opened by the recognition probe. The nicked double helices from hybridization chain reaction are used to immobilize horseradish peroxidase enzymes via biotin–avidin reaction, which produces signal-amplification detection of target DNA through the catalytic reaction of the hydrogenperoxide + hydroquinone system. Under optimum conditions, the as-prepared biosensor shows a good linear relationship between the current value and logarithm of the target DNA concentration ranging from 0.001 pM to 100 pM and a detection limit as low as 0.12 fM. Moreover, the fabricated biosensor exhibits good selectivity to differentiate the one-base mismatched DNA sequence. This work will open a pathway for ultrasensitive detection of other biorecognition events and gene-related diseases based on layered WS2–AB and hybridization chain reaction.