CXCR5-dependent seeding of follicular niches by B and Th cells augments antiviral B cell responses
CXCR5-dependent seeding of follicular niches by B and Th cells augments antiviral B cell responses
复制标题
DOI：
10.4049/jimmunol.175.11.7109
复制
发表时间：
2005-12-01
影响因子：
4.4
通讯作者：
Hengartner, H
中科院分区：
文献类型：
--
作者：
Junt, T;Fink, K;Hengartner, H
趋化因子受体CXCR5及其配体CXCL13定义了继发性淋巴机构内B细胞卵泡的结构。在这里，我们检查了CXCR5对体内抗病毒B细胞反应的影响。 CXCR5（ - / - ）小鼠在用囊泡口腔炎病毒（VSV）感染后急性地产生IgM和IgG，并发展为VSV特异性生发中心。然而，在AG的可用性有限的条件下（即，用不复制的病毒颗粒或可溶性AG进行免疫后，IG类开关和AB产生受损）。 CXCR5缺陷，VSV特异性B和TH细胞的过继转移表明，有效的Ig类开关需要B和TH细胞上的CXCR5表达。这些实验表明，CXCR5通过其对初始B细胞膨胀以及Ag特异性B和TH细胞对生发中心的影响，对于抗病毒T和B细胞的协调相互作用至关重要。
The chemokine receptor CXCR5 and its ligand CXCL13 define the structure of B cell follicles within secondary lymphoid organs. Here, we examined the impact of CXCR5 on antiviral B cell responses in vivo. CXCR5(-/-) mice showed a normal production of IgM and IgG acutely after infection with vesicular stomatitis virus (VSV) and developed VSV-specific germinal centers. However, impaired Ig class switch and Ab production were observed under conditions of limited availability of Ag (i.e., after immunization with nonreplicating viral particles or soluble Ag). Adoptive transfer of CXCR5-deficient, VSV-specific B and Th cells demonstrated that CXCR5 expression on both B and Th cells is required for an efficient Ig class switch. These experiments revealed that CXCR5 is critical for the coordinated interaction of antiviral T and B cells through its impact on initial B cell expansion and the recruitment of Ag-specific B and Th cells to germinal centers.