Activated protein C resistance in ischemic stroke not due to factor V arginine506-->glutamine mutation.
Activated protein C resistance in ischemic stroke not due to factor V arginine506-->glutamine mutation.
复制标题
缺血性中风中激活的蛋白 C 抵抗并非由于因子 V 精氨酸 506--> 谷氨酰胺突变。
DOI：
10.1161/01.str.27.7.1163
复制
发表时间：
1996
期刊：
影响因子：
8.3
通讯作者：
Griffin,JH
中科院分区：
文献类型：
--
作者：
Fisher,M;Fernandez,JA;Ameriso,SF;Xie,D;Gruber,A;Paganini-Hill,A;Griffin,JH
背景和目的对活化蛋白C（APC）的抵抗是静脉血栓栓塞性疾病最常见的危险因素。在>90%的APC耐药静脉血栓形成患者中发现凝血因子V的一个点突变，使其对APC耐药。为了确定APC抵抗和中风中的这种因子V突变的患病率，我们筛选了一组缺血性中风patients.MethodsHispanic缺血性中风患者筛选使用两种不同的活化部分凝血活酶时间为基础的测定。一项试验使用纯患者血浆测定APC耐药，另一项试验使用稀释至因子V缺陷血浆中的患者血浆测定APC耐药因子V，包括Arg506→Gln突变。结果进行了比较，在31个西班牙裔对照组的相似ages.Results6 63（9.5%）中风患者APC阻力相比，没有31（0%）的对照组。没有患者或对照组有APC耐药因子V，即因子V Arg506→Gln mutation.ConclusionsIn西班牙裔缺血性卒中患者，APC耐药的发病率（约10%）是不是由因子V Arg506→Gln突变引起的。在该人群中，非由该因子V突变引起的APC抵抗可能是缺血性卒中的危险因素。
Background and PurposeResistance to activated protein C (APC), a natural plasma anticoagulant, is the most common identifiable risk factor for venous thromboembolic disease. One point mutation in coagulation factor V that renders it APC-resistant is found in >90% of APC-resistant venous thrombosis patients. To determine the prevalence of APC resistance and of this factor V mutation in stroke, we screened a group of ischemic stroke patients.MethodsHispanic ischemic stroke patients were screened using two different activated partial thromboplastin time-based assays. One assay using neat patient plasma determined APC resistance, and the other assay using patient plasma diluted into factor V-deficient plasma determined APC-resistant factor V, including the Arg506→Gln mutation. Results were compared with those in 31 Hispanic control subjects of similar ages.ResultsSix of 63 (9.5%) stroke patients had APC resistance compared with none of 31 (0%) control subjects. No patient or control subject had APC-resistant factor V, ie, the factor V Arg506→Gln mutation.ConclusionsIn Hispanic patients with ischemic stroke, the incidence (approximately 10%) of APC resistance is not caused by the factor V Arg506→Gln mutation. APC resistance not caused by this factor V mutation may be a risk factor for ischemic stroke in this population.