Using decoys to expand the recognition specificity of a plant disease resistance protein
Using decoys to expand the recognition specificity of a plant disease resistance protein
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DOI：
10.1126/science.aad3436
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发表时间：
2016-02-12
期刊：
影响因子：
56.9
通讯作者：
Innes, Roger W.
中科院分区：
文献类型：
--
作者：
Kim, Sang Hee;Qi, Dong;Innes, Roger W.
以环境可持续的方式保持作物高产需要开发抗病作物品种。我们描述了一种利用拟南芥内源性抗病基因RPS5来设计植物抗病的方法，该基因编码核苷酸结合富亮氨酸重复（NLR）蛋白。当第二宿主蛋白PBS1被病原体分泌的蛋白酶AvrPphB切割时，RPS5通常会被激活。我们发现，PBS1中的AvrPphB切割位点可以被其他病原体蛋白酶的切割位点取代，这使得RPS5能够被这些蛋白酶激活，从而赋予对新病原体的抗性。这种“诱饵”方法可能适用于其他NLR蛋白，并且应该能够使我们目前缺乏强大遗传抗性的植物抗病工程成为可能。
Maintaining high crop yields in an environmentally sustainable manner requires the development of disease-resistant crop varieties. We describe a method to engineer disease resistance in plants by means of an endogenous disease resistance gene from Arabidopsis thaliana named RPS5, which encodes a nucleotide-binding leucine-rich repeat (NLR) protein. RPS5 is normally activated when a second host protein, PBS1, is cleaved by the pathogen-secreted protease AvrPphB. We show that the AvrPphB cleavage site within PBS1 can be substituted with cleavage sites for other pathogen proteases, which then enables RPS5 to be activated by these proteases, thereby conferring resistance to new pathogens. This "decoy" approach may be applicable to other NLR proteins and should enable engineering of resistance in plants to diseases for which we currently lack robust genetic resistance.