Functional conservation of mouse Notch receptor family members
Functional conservation of mouse Notch receptor family members
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DOI：
10.1016/0014-5793(96)01046-0
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发表时间：
1996-10-21
期刊：
影响因子：
3.5
通讯作者：
Honjo, T
中科院分区：
文献类型：
--
作者：
Kato, H;Sakai, T;Honjo, T
通过与DNA结合蛋白RBP-J κ的相互作用来检查小鼠Notch受体家族的所有已知成员的生化功能。通过GST融合下拉测定和与爱泼斯坦巴尔病毒核抗原2的显性负竞争，mNotch 2、mNotch 3和int 3（= mNotch 4）仅显示与RBP-J κ相互作用。此外，int 3的细胞内区域显示反式激活Epstein巴尔病毒TP 1启动子。这些结果表明，所有小鼠Notch家族成员都具有与mNotch 1相似的生化功能，mNotch 1通过与DNA结合蛋白RBP-J κ直接相互作用来转导增殖信号。
All the known members of the mouse Notch receptor family were examined for their biochemical function by interaction with a DNA binding protein RBP-J kappa. mNotch2, mNotch3 and int3 (=mNotch4) mere shown to interact with RBP-J kappa by the GST-fusion pull down assay and dominant negative competition with Epstein Barr virus nuclear antigen 2. Furthermore the intracellular region of int3 was shown to transactivate the Epstein Barr virus TP1 promoter. These results indicate that all mouse Notch family members have biochemical functions similar to mNotch1, which transduces proliferative signal by direct interaction with the DNA binding protein RBP-J kappa.