Experimental alveolar echinococcosis in pigs, lesion development and serological follow up
Experimental alveolar echinococcosis in pigs, lesion development and serological follow up
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DOI：
10.1016/j.vetpar.2005.03.034
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发表时间：
2005-06-30
影响因子：
2.6
通讯作者：
Kapel, CMO
中科院分区：
文献类型：
--
作者：
Deplazes, P;Grimm, F;Kapel, CMO
经口接种5000或35，000个多房棘球绦虫卵后7个月，6/6头猪出现肝脏病变。然而，动物之间的病变形态差异很大。最大病变（直径3-8 mm）见于1头猪，较小病变（1.5-3 mm）见于3头猪。这些病变界限清楚，有明显的中央坏死和营养不良性钙化。相比之下，在另外两只猪中发现的大多数最小（直径通常< 1.5 mm），具有小的致密纤维化区域和边界模糊，小叶间组织中有明显的纤维浸润。E.在所有的病变类型中，通过PCR检测到多房虫特异性DNA，但通过在沙鼠体内腹腔内接种评估的后绦虫活力，不能证明。在接种后1个月内，所有猪都产生了针对一系列不同抗原（后绦虫、囊液和原绦虫衍生的天然E. multilocularis和E.颗粒体抗原、亲和纯化的Em 2 G11抗原、抗原B、重组Em II/3-10抗原）。记录了两种不同的反应模式。在具有小病变的两头猪中，对所有粗抗原的明显反应在感染后3-5个月达到峰值。并且直到实验结束时水平明显升高。在所有其他猪中，抗体反应在所有情况下均保持较低水平。总之，我们证明了两种类型的E。猪的多房囊蚴发育具有不同的免疫反应模式。(c)2005 Elsevier B. V.保留所有权利。
Liver lesions were found in 6/6 pigs 7 months after oral inoculation with 5000 or 35,000 Echinococcus multilocularis eggs. However, lesion morphology differed considerably among the animals. The largest lesions (3-8 mm in diameter) were found in a single pig and smaller lesions (1.5-3 mm) in three pigs. These lesions were clearly circumscribed and had pronounced central necroses and dystrophic calcifications. In contrast, most of the smallest (usually < 1.5 mm in diameter) found in two other pigs, had small compact fibrotic areas and blurred borders with obvious fibrous infiltrations into the interlobular tissues. E. multilocularis specific DNA was detected by PCR in all lesion types, but metacestode viability, as assessed by in vivo intraperitoneal inoculations in jirds, could not be demonstrated. Within I month post inoculation, all pigs developed specific IgG antibody responses against a battery of different antigens (metacestode, cyst fluid, and protoscoleces-derived native E. multilocularis and E. granulosus antigens, affinity purified Em2G11 antigen, antigen B, recombinant Em II/3-10 antigen). Two different reaction patterns were recorded. In the two pigs with the small lesions, pronounced reactions against all crude antigens with peaks 3-5 months p.i. and clearly elevated levels until the end of the experiment were noted. In all other pigs, antibody reactions remained low in all cases. In conclusion, we demonstrated two types of E. multilocularis metacestode development in pigs with distinct immunological response patterns. (c) 2005 Elsevier B.V. All rights reserved.