Voluntary exercise and clomipramine treatment elevate prepro-galanin mRNA levels in the locus coeruleus in rats
Voluntary exercise and clomipramine treatment elevate prepro-galanin mRNA levels in the locus coeruleus in rats
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DOI：
10.1016/j.neulet.2006.04.057
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发表时间：
2006-11-06
影响因子：
2.5
通讯作者：
Dishman, Rod K.
中科院分区：
文献类型：
--
作者：
Holmes, Philip V.;Yoo, Ho Sang;Dishman, Rod K.
运动在人类和情感障碍的啮齿动物模型中发挥抗抑郁作用。这些作用可能是通过上调发挥抗抑郁作用的内源性因素来实现的。神经肽甘丙肽(Galanin，GAL)的生理功能和行为行为提示其具有抗抑郁活性。以往的研究表明，不同的运动方式提高了大鼠蓝斑(LC)GAL基因的表达。本实验研究了自愿锻炼和抗抑郁药物治疗之间的相互作用。雄性SD大鼠在28天内接触活动轮(运动状态)或非活动轮(久坐状态)。在此期间，各组大鼠均注射氯丙咪嗪(10 mg/kg/d)或赋形剂(连续3周)。用原位杂交组织化学方法检测LC中Pre-Gal基因的表达。运动和氯丙咪嗪治疗显著提高了Gal基因的表达，尽管接受这两种干预的大鼠的Gal前基因表达水平与接受药物的久坐对照组没有区别。GAL前基因表达水平与跑步距离显著相关。这一结果进一步暗示了GAL在运动和理疗的抗抑郁作用中的作用，尽管这些治疗影响GAL基因表达的机制似乎有很大不同。(C)2006爱思唯尔爱尔兰有限公司。保留所有权利。
Exercise exerts antidepressant effects in humans and rodent models of affective disorders. These effects may be mediated by the upregulation of endogenous factors that exert antidepressant actions. The physiological functions and behavioral actions of the neuropeptide galanin (GAL) suggest antidepressant activity. Previous studies have shown that various modes of exercise elevate GAL gene expression in the locus coeruleus (LC) in rats. The present experiments examined the interaction between voluntary exercise and antidepressant pharmacotherapy. Male Sprague-Dawley rats were provided access to activity wheels (exercise condition) or inoperative wheels (sedentary condition) for 28 days. Rats in each group were injected with clomipramine (10 mg/kg/day) or vehicle throughout this period (for 3 weeks). Prepro-GAL mRNA in the LC was measured by in situ hybridization histochemistry. Exercise and clomipramine treatment significantly elevated GAL gene expression, though prepro-GAL mRNA levels in rats receiving both interventions did not differ from sedentary controls that received vehicle. Prepro-GAL mRNA levels were significantly correlated with running distance. The results further implicate a role for GAL in the antidepressant effects of exercise and pharmaeotherapy, though the mechanisms through which these treatments influence GAL gene expression appear to differ significantly. (c) 2006 Elsevier Ireland Ltd. All rights reserved.