A Simple Method for the Detection of Long-Chain Fatty Acids in an Anaerobic Digestate Using a Quartz Crystal Sensor
A Simple Method for the Detection of Long-Chain Fatty Acids in an Anaerobic Digestate Using a Quartz Crystal Sensor
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DOI：
10.3390/en10010019
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发表时间：
2016-12
期刊：
影响因子：
3.2
通讯作者：
Takurou Kobayashi;H. Kuramochi;K. Maeda;Kaiqin Xu
中科院分区：
文献类型：
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作者：
Takurou Kobayashi;H. Kuramochi;K. Maeda;Kaiqin Xu
在厌氧消化过程中，脂肪水解产生的长链脂肪酸（LCFAs）当其浓度超过几个毫摩尔时会对微生物产生毒性。利用石英晶体微天平（QCM）建立了一种吸附检测系统，用于监测含油有机废物厌氧消化过程中LCFA的浓度。LCFA的解离大大提高了传感器的响应，而且，使其能够从LCFA和甘油三酯的混合物中特异性地检测LCFA。在碱性条件下，在0-100 mg/L范围内，QCM传感器的频移速率与棕榈酸浓度呈线性关系。在去除悬浮固体并将pH调节至10.7后，成功地测量了由厌氧厌氧消化状态样品引起的频率变化。最后，QCM测量结果表明，频率偏移率与LCFA浓度高度相关，这证实了新开发的QCM传感器有助于LCFA监测的快速性和可用性。
In anaerobic digestion (AD), long-chain fatty acids (LCFAs) produced by hydrolysis of lipids, exhibit toxicity against microorganisms when their concentration exceeds several millimolar. An absorption detection system using a quartz crystal microbalance (QCM) was developed to monitor the LCFA concentration during an anaerobic digester’s operation treating oily organic waste. The dissociation of the LCFAs considerably improved the sensor response and, moreover, enabled it to specifically detect LCFA from the mixture of LCFA and triglyceride. Under alkaline conditions, the frequency-shift rates of the QCM sensor linearly increased in accordance with palmitic acid concentration in the range of 0–100 mg/L. Frequency changes caused by anaerobic digestate samples were successfully measured after removing suspended solids and adjusting the pH to 10.7. Finally, the QCM measurements for digestate samples demonstrated that frequency-shift rates are highly correlated with LCFA concentrations, which confirmed that the newly developed QCM sensor is helpful for LCFA monitoring in terms of rapidness and usability.