Time and concentration dependent interactions of Beauveria bassiana with sublethal rates of imidacloprid against the aphid pests Macrosiphoniella sanborni and Myzus persicae
Time and concentration dependent interactions of Beauveria bassiana with sublethal rates of imidacloprid against the aphid pests Macrosiphoniella sanborni and Myzus persicae
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DOI：
10.1111/j.1744-7348.2005.040147.x
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发表时间：
2005-01-01
影响因子：
2.6
通讯作者：
Feng, MG
中科院分区：
文献类型：
--
作者：
Ye, SD;Dun, YH;Feng, MG
采用低、中、高浓度的球孢白僵菌（Beauveria bassiana）单独处理或添加亚致死率递增的吡虫啉（imidacloprid）处理，对菊蚜Macrosiphoniella sanborni（0.01-0.05 a.i.）μ g mL（-1））和绿色桃蚜Myzus persicae（0.05-0.5 a.i.μ g mL（-1））。在暴露于波特喷雾塔中的95 cm（2）面积上的1 mL喷雾后一周内，M。sanborni要么对B更敏感。白僵菌对吡虫啉的敏感性高于桃蚜。时间-浓度-死亡率（TCM）的响应，每种蚜虫在每五个生物测定符合TCM模型，表明浓度和喷雾后时间的真菌和化学相互作用的强烈依赖。在B中加入吡虫啉。白僵菌以0.025-0.05 μ g·mL ~（-1）的浓度对M. sanborni或0.1-0.5 μ g mL（-1）对桃蚜的抑菌作用显著增强或加速。根据LC 50或LC 90估计值及其方差（由拟合的TCM关系确定），计算了含吡虫啉的生物测定法相对于含B的生物测定法的相对效力。单独或与较低亚致死率一起使用时，亚致死率范围从几倍到几百倍不等，并在喷雾后的几天内变化。这些结果表明，通过组合制剂或施用B，实际控制蚜虫害虫的替代策略。并治理蚜虫对化学杀虫剂的抗性。
A series of laboratory bioassays with each consisting of low, medium and high concentration treatments of the fungal biocontrol agent Beauveria bassiana alone or supplemented with an increasing sublethal rate of imidacloprid were conducted to quantify the fungal and chemical interactions on chrysanthemum aphid Macrosiphoniella sanborni (0.01-0.05 a.i. mu g mL(-1)) and green peach aphid Myzus persicae (0.05-0.5 a.i. mu g mL(-1)). During one week after exposure to a 1 mL spray onto a 95 cm(2) area in a Potter Spray Tower, M. sanborni was either more susceptible to B. bassiana or more sensitive to imidacloprid than M persicae. The time-concentration-mortality (TCM) responses of each aphid species in each of five bioassays fit well to a TCM model, indicating a strong dependence of the fungal and chemical interactions on both concentration and post-spray time. Adding imidacloprid to B. bassiana sprays at the rates of 0.025-0.05 mu g mL(-1) against M. sanborni or 0.1-0.5 mu g mL(-1) against M persicae significantly enhanced or accelerated the fungal action. Based on the LC50 or LC90 estimates and their variances determined by the fitted TCM relationships for each aphid species, the relative potencies of an imidacloprid-inclusive bioassay over those with B. bassiana alone or together with a lower sublethal rate ranged from a few to hundreds of times and varied over days after spray. These results suggest an alternative tactic for practical control of the aphid pests by a combined formulation or application of B. bassiana and imidacloprid and manage aphid resistance to the chemical insecticide.