Prostaglandin E2 facilitates neurite outgrowth in a motor neuron-like cell line, NSC-34
Prostaglandin E2 facilitates neurite outgrowth in a motor neuron-like cell line, NSC-34
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DOI：
10.1016/j.jphs.2017.09.001
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发表时间：
2017-10-01
影响因子：
3.5
通讯作者：
Ishige, Kumiko
中科院分区：
文献类型：
--
作者：
Nango, Hiroshi;Kosuge, Yasuhiro;Ishige, Kumiko
前列腺素E2（PGE 2）通过与E-前列腺素受体（EP 1 -4）结合发挥各种生物学作用。虽然最近的研究表明，PGE 2诱导一些神经元细胞，如小鼠DRG神经元和感觉神经元样ND 7/23细胞的细胞分化，但尚不清楚PGE 2是否在运动神经元的分化中发挥作用。在本研究中，我们研究了PGE 2诱导运动神经元分化的机制，使用NSC-34，一个小鼠运动神经元样细胞系。未分化的NSC-34细胞暴露于PGE 2和EP 2选择性激动剂布他前列素，导致MTT还原活性降低，而碘化丙啶阳性细胞的数量没有增加，神经突承载细胞的数量增加。EP 1/3激动剂Sulprostone也显著降低MTT还原活性20%;然而，在测试的浓度范围内未观察到神经突承载细胞数量的增加。PGE 2诱导的轴突生长在PF-0441848（一种EP 2选择性拮抗剂）的存在下显著减弱。用二丁酰-cAMP处理这些细胞增加了神经突承载细胞的数量，而对细胞增殖没有影响。这些结果表明，PGE 2通过激活EP 2亚型促进神经突起生长并抑制细胞增殖，并且cAMP信号通路参与PGE 2诱导的NSC-34细胞分化。(C)2017年，作者。Elsevier B. V.代表日本药理学会制作和主办。
Prostaglandin E2 (PGE2) exerts various biological effects by binding to E-prostanoid receptors (EP1-4). Although recent studies have shown that PGE2 induces cell differentiation in some neuronal cells such as mouse DRG neurons and sensory neuron-like ND7/23 cells, it is unclear whether PGE2 plays a role in differentiation of motor neurons. In the present study, we investigated the mechanism of PGE2-induced differentiation of motor neurons using NSC-34, a mouse motor neuron-like cell line. Exposure of undifferentiated NSC-34 cells to PGE2 and butaprost, an EP2-selective agonist, resulted in a reduction of MTT reduction activity without increase the number of propidium iodide-positive cells and in an increase in the number of neurite-bearing cells. Sulprostone, an EP1/3 agonist, also significantly lowered MTT reduction activity by 20%; however, no increase in the number of neurite-bearing cells was observed within the concentration range tested. PGE2-induced neurite outgrowth was attenuated significantly in the presence of PF-0441848, an EP2-selective antagonist. Treatment of these cells with dibutyryl-cAMP increased the number of neurite-bearing cells with no effect on cell proliferation. These results suggest that PGE2 promotes neurite outgrowth and suppresses cell proliferation by activating the EP2 subtype, and that the cAMP-signaling pathway is involved in PGE2-induced differentiation of NSC-34 cells. (C) 2017 The Authors. Production and hosting by Elsevier B.V. on behalf of Japanese Pharmacological Society.