Protective effect of L-carnitine against cisplatin-induced liver and kidney oxidant injury in rats
Protective effect of L-carnitine against cisplatin-induced liver and kidney oxidant injury in rats
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DOI：
10.2478/s11536-009-0021-x
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发表时间：
2009-06-01
影响因子：
--
通讯作者：
Tekin, Salim Basol
中科院分区：
文献类型：
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作者：
Cayir, Kerim;Karadeniz, Ali;Tekin, Salim Basol
观察L肉碱对顺铂所致大鼠肝肾组织脂质过氧化及内源性抗氧化剂水平变化的保护作用。将24只SD大鼠平均分为4组：对照组、顺铂组、L-卡尼汀组和L-卡尼汀+顺铂组，每组6只。通过测定丙二醛含量来评价L肉碱的保护作用。测定肝、肾组织匀浆中谷胱甘肽、谷胱甘肽过氧化物酶、谷胱甘肽S转移酶和超氧化物歧化酶的活性，并对肝、肾进行组织学检查。L-卡尼汀通过降低脂质过氧化水平，提高GSH、GSHPx、GST、SOD活性，具有明显的肝肾保护作用。此外，这些生化观察得到了组织学结果的支持。综上所述，本研究表明活性氧自由基(ROS)在肝肾损害中的重要作用及其与肝肾功能损害的关系，并指出LC在顺铂所致肝肾毒性中的治疗潜力。
The present study was designed to investigate the protective effects of L-carnitine (LC) on changes in the levels of lipid peroxidation and endogenous antioxidants induced by cisplatin (cis-diamminedichloroplatinum II, CDDP) in the liver and kidney tissues of rats. Twenty-four Sprague Dawley rats were equally divided into four groups of six rats each: control, cisplatin, L-carnitine, and L-carnitine plus cisplatin. The degree of protection produced by L-carnitine was evaluated by determining the level of malondialdehyde (MDA). The activity of glutathione (GSH), glutathione peroxidase (GSH-Px), glutathione S-transferase (GST), and superoxide dismutase (SOD) were estimated from liver and kidney homogenates, and the liver and kidney were histologically examined as well. L-carnitine elicited significant liver and kidney protective activity by decreasing the level of lipid peroxidation (MDA) and elevating the activity of GSH, GSHPx, GST, and SOD. Furthermore, these biochemical observations were supported by histological findings. In conclusion, the present study indicates a significant role for reactive oxygen species (ROS) and their relation to liver and kidney dysfunction, and points to the therapeutic potential of LC in CDDP-induced liver and kidney toxicity.