Design and characterization of nanoknife with buffering beam for in situ single-cell cutting
Design and characterization of nanoknife with buffering beam for in situ single-cell cutting
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DOI：
10.1088/0957-4484/22/30/305701
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发表时间：
2011-07
期刊：
影响因子：
3.5
通讯作者：
Yajing Shen;M. Nakajima;Zhan Yang;S. Kojima;M. Homma;T. Fukuda
中科院分区：
文献类型：
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作者：
Yajing Shen;M. Nakajima;Zhan Yang;S. Kojima;M. Homma;T. Fukuda
提出了一种用于单细胞切割的具有缓冲梁的新型纳米刀。纳米刀是由商业原子力显微镜（AFM）的悬臂梁通过聚焦离子束（FIB）蚀刻技术。使用能量色散谱（EDS）方法确定纳米刀的材料鉴定。结果表明，在刻蚀过程中，镓离子对纳米刀具的污染可以忽略不计。利用缓冲梁的变形测量切削力。基于参考悬臂梁，通过使用纳米操纵方法校准梁的弹簧常数。纳米刀的尖端被设计成具有5°的小刃角，以减少切割过程中对细胞的压缩。为了比较，还制备了具有不同棱角（即25°和45°）的两种其他纳米刀。在环境扫描电子显微镜（ESEM）内使用这三种纳米刀进行原位单细胞切割实验。评估每个纳米刀的切削力和样品切片角度。这表明当使用具有5°小刃角的纳米刀时，可以减小对细胞的压缩。因此，纳米刀能够进行原位单细胞切割任务。
A novel nanoknife with a buffering beam is proposed for single-cell cutting. The nanoknife was fabricated from a commercial atomic force microscopy (AFM) cantilever by focused-ion-beam (FIB) etching technique. The material identification of the nanoknife was determined using the energy dispersion spectrometry (EDS) method. It demonstrated that the gallium ion pollution of the nanoknife can be ignored during the etching processes. The buffering beam was used to measure the cutting force based on its deformation. The spring constant of the beam was calibrated based on a referenced cantilever by using a nanomanipulation approach. The tip of the nanoknife was designed with a small edge angle 5° to reduce the compression to the cell during the cutting procedure. For comparison, two other nanoknives with different edge angles, i.e. 25° and 45°, were also prepared. An in situ single-cell cutting experiment was performed using these three nanoknives inside an environmental scanning electron microscope (ESEM). The cutting force and the sample slice angle for each nanoknife were evaluated. It showed the compression to the cell can be reduced when using the nanoknife with a small edge angle 5°. Consequently, the nanoknife was capable for in situ single-cell cutting tasks.