Extraction of DNA from Plant Tissue: Review and Protocols

Extraction of DNA from Plant Tissue: Review and Protocols
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DOI：
10.1007/978-1-4939-3185-9_17
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发表时间：
2016
期刊：
--
影响因子：
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通讯作者：
F. Tamari;C. Hinkley
F. Tamari;C. Hinkley
中科院分区：
其他
文献类型：
--
作者：
F. Tamari;C. Hinkley

文献摘要

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DNA提取通常是大多数分子生物学研究的第一步。DNA可以从任何生物体和各种组织中提取，并取得不同程度的成功。本章重点介绍植物DNA的提取。本文根据植物的分类、科、种、使用的组织以及提取的DNA的应用，为研究人员提供了一份有用的参考清单。我们讨论了从不同的植物组织中提取DNA，以及一些下游应用，其中分离的DNA是使用。我们还列出了一些用于DNA提取的市售试剂盒。最后，我们给出了两种详细的提取方案，一种是CTAB法，另一种是Edwards法。
DNA extraction is usually the first step required for most molecular biology investigations. DNA can be extracted from any organism and from a variety of tissues with varying degrees of success. In this chapter we focus on DNA extraction from plants. We provide a useful reference list for researchers summarizing investigations that have used DNA extraction categorized by plant division, family, species, tissues used, and the application for the extracted DNA. We discuss extraction of DNA from different plant tissues as well as some of the downstream application for which the isolated DNA is used. We also list some of the kits that are commercially available for DNA extraction. Finally, we provide two detailed extraction protocols, one using the CTAB method and the other using the Edwards method.