Transgenic labeling of the corticospinal tract for monitoring axonal responses to spinal cord injury
Transgenic labeling of the corticospinal tract for monitoring axonal responses to spinal cord injury
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DOI：
10.1038/nm1331
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发表时间：
2005-12-01
期刊：
影响因子：
82.9
通讯作者：
Sanes, JR
中科院分区：
文献类型：
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作者：
Bareyre, FM;Kerschensteiner, M;Sanes, JR
啮齿动物皮质脊髓束(CST)已被广泛用于研究损伤后中枢轴突的再生和重塑。目前CST轴突是在注射示踪染料后可见的，示踪染料具有侵入性、不完全和易变异的特点，通常不显示功能上关键的但数量上较小的束组件。在这里，我们描述了CST纤维被黄色荧光蛋白(YFP)特异性和完全标记的转基因小鼠。利用这些CST-YFP小鼠，我们发现CST的微小成分负责大多数单突触与运动神经元的接触。病变的主要背侧CST导致新的侧支延伸，其中一些起源于小的背外侧和腹侧CST成分内的大的、大量有髓鞘的轴突。其中一些新的侧支与运动神经元形成额外的直接突触。我们认为，CST-YFP小鼠将有助于评估旨在最大化这种重塑和促进再生的策略。
The rodent corticospinal tract (CST) has been used extensively to investigate regeneration and remodeling of central axons after injury. CST axons are currently visualized after injection of tracer dye, which is invasive, incomplete and prone to variation, and often does not show functionally crucial but numerically minor tract components. Here, we characterize transgenic mice in which CST fibers are specifically and completely labeled by yellow fluorescent protein (YFP). Using these CST-YFP mice, we show that minor CST components are responsible for most monosynaptic contacts onto motoneurons. Lesions of the main dorsal CST lead to extension of new collaterals, some of them originating from large, heavily myelinated axons within the minor dorsolateral and ventral CST components. Some of these new collaterals form additional direct synapses onto motoneurons. We propose that CST-YFP mice will be useful for evaluating strategies designed to maximize such remodeling and to promote regeneration.