Brain substrates activated by electroacupuncture (EA) of different frequencies .2. Role of Fos/Jun proteins in EA-induced transcription of preproenkephalin and preprodynorphin genes
Brain substrates activated by electroacupuncture (EA) of different frequencies .2. Role of Fos/Jun proteins in EA-induced transcription of preproenkephalin and preprodynorphin genes
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DOI：
10.1016/s0169-328x(96)00171-4
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发表时间：
1996-12-31
期刊：
影响因子：
--
通讯作者：
Han, JS
中科院分区：
文献类型：
--
作者：
Guo, HF;Tian, JH;Han, JS
本研究采用c-fos和/或c-jun的反义寡核苷酸（ODNs），探讨了Fos和Jun蛋白在电针诱导阿片基因前脑啡肽原（PPE）、前强啡肽原（PPD）和前阿黑皮素（POMC）转录中的作用。结果表明，c-fos和c-jun反义寡核苷酸分别有效、特异地阻断了EA诱导的Fos和Jun表达。这种处理显著阻止EA诱导的PPD，但不PPE，mRNA表达。这些结果表明，Fos和Jun蛋白参与了EA刺激激活的PPD而不是PPE基因转录。
Antisense oligodeoxynucleotides (ODNs) of c-fos and/or c-jun were used in this study to investigate the role of Fos and Jun proteins in electroacupuncture (EA)-induced transcription of the opioid genes preproenkephalin (PPE), preprodynorphin (PPD) and proopiomelanocortin (POMC). As the results showed, EA-induced Fos and Jun expression was blocked efficiently and specifically by c-fos and c-jun antisense ODNs, respectively. This treatment significantly prevented EA-induced PPD, but not PPE, mRNA expression. These results suggest that Fos and Jun proteins are involved in PPD rather than PPE gene transcription activated by EA stimulation.