Structural analysis of herpes simplex virus by optical super-resolution imaging.
Structural analysis of herpes simplex virus by optical super-resolution imaging.
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DOI：
10.1038/ncomms6980
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发表时间：
2015-01-22
影响因子：
16.6
通讯作者：
Kaminski, Clemens F.
中科院分区：
文献类型：
--
作者：
Laine, Romain F.;Albecka, Anna;van de Linde, Sebastian;Rees, Eric J.;Crump, Colin M.;Kaminski, Clemens F.
单纯疱疹病毒1型(HSV-1)是人类传播最广泛的病原体之一。尽管对HSV-1的结构进行了广泛的研究，但对被膜和包膜蛋白的确切组织仍然难以捉摸。在这里，我们使用直接随机光学重建显微镜(DSTORM)的超分辨率成像，并结合基于模型的单分子定位数据分析，来确定病毒颗粒中蛋白质层的位置。我们使用多色dSTORM成像技术解析单个HSV-1颗粒内的不同蛋白质层，并区分纯化的病毒粒子和感染细胞中存在的病毒粒子中的包膜锚定糖蛋白和被膜蛋白。通过对纯化病毒的颗粒平均和基于模型的分析被膜蛋白VP16、VP1/2和pUL37以及包膜蛋白gD的径向分布，实现了对HSV-1结构的精确表征。根据这些数据，我们提出了一个关于被膜内蛋白质组织的模型。本文的在线版本(doi：10.1038/nComms6980)包含补充材料，授权用户可以使用。单纯疱疹病毒1型(HSV-1)是一种分布广泛的人类病原体，其结构尚未完全确定。在这里，作者应用dSTORM超分辨率显微镜结合先进的数据分析工具，以前所未有的精度定位HSV-1中四个关键蛋白质层的位置。本文的在线版本(doi：10.1038/nComms6980)包含补充材料，授权用户可以使用。
Herpes simplex virus type-1 (HSV-1) is one of the most widespread pathogens among humans. Although the structure of HSV-1 has been extensively investigated, the precise organization of tegument and envelope proteins remains elusive. Here we use super-resolution imaging by direct stochastic optical reconstruction microscopy (dSTORM) in combination with a model-based analysis of single-molecule localization data, to determine the position of protein layers within virus particles. We resolve different protein layers within individual HSV-1 particles using multi-colour dSTORM imaging and discriminate envelope-anchored glycoproteins from tegument proteins, both in purified virions and in virions present in infected cells. Precise characterization of HSV-1 structure was achieved by particle averaging of purified viruses and model-based analysis of the radial distribution of the tegument proteins VP16, VP1/2 and pUL37, and envelope protein gD. From this data, we propose a model of the protein organization inside the tegument. The online version of this article (doi:10.1038/ncomms6980) contains supplementary material, which is available to authorized users. Herpes simplex virus type-1 (HSV-1) is a widespread human pathogen, the structure of which is not yet fully characterized. Here, the authors apply dSTORM super-resolution microscopy in combination with advanced data analysis tools to locate the position of four key protein layers in HSV-1 with unprecedented precision. The online version of this article (doi:10.1038/ncomms6980) contains supplementary material, which is available to authorized users.
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影响因子：
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作者：
Ivanchenko S;Godinez WJ;Lampe M;Kräusslich HG;Eils R;Rohr K;Bräuchle C;Müller B;Lamb DC
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Lamb DC
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影响因子：
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作者：
Ehmann, Nadine;van de Linde, Sebastian;Alon, Amit;Ljaschenko, Dmitrij;Keung, Xi Zhen;Holm, Thorge;Rings, Annika;DiAntonio, Aaron;Hallermann, Stefan;Ashery, Uri;Heckmann, Manfred;Sauer, Markus;Kittel, Robert J.
通讯作者：
Kittel, Robert J.